Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PotMrB4_RS05475 Genome accession   NZ_AP022642
Coordinates   1163641..1165236 (-) Length   531 a.a.
NCBI ID   WP_074973001.1    Uniprot ID   A0A1I0USM3
Organism   Metapseudomonas otitidis strain MrB4     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1158641..1170236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS05460 (PtoMrB4_10890) - 1160727..1161140 (+) 414 WP_172432730.1 type IV pilin protein -
  PotMrB4_RS05465 (PtoMrB4_10900) thiO 1161191..1162285 (+) 1095 WP_172432731.1 glycine oxidase ThiO -
  PotMrB4_RS05470 (PtoMrB4_10910) pilR 1162298..1163638 (-) 1341 WP_044410357.1 sigma-54 dependent transcriptional regulator Regulator
  PotMrB4_RS05475 (PtoMrB4_10920) pilS 1163641..1165236 (-) 1596 WP_074973001.1 PAS domain-containing sensor histidine kinase Regulator
  PotMrB4_RS05480 (PtoMrB4_10930) - 1165223..1165459 (-) 237 WP_172432732.1 PP0621 family protein -
  PotMrB4_RS05485 (PtoMrB4_10940) - 1165606..1166604 (-) 999 WP_044410365.1 outer membrane protein assembly factor BamD -
  PotMrB4_RS05490 (PtoMrB4_10950) rluD 1166752..1167723 (+) 972 WP_044410847.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PotMrB4_RS05495 (PtoMrB4_10960) pgeF 1167723..1168448 (+) 726 WP_172432733.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 59155.37 Da        Isoelectric Point: 8.3605

>NTDB_id=79057 PotMrB4_RS05475 WP_074973001.1 1163641..1165236(-) (pilS) [Metapseudomonas otitidis strain MrB4]
MASTESPSLASNQIRRIFRLYNLYRLLIGLALVGLISSELDSELLNLARVQLFRNASWFYLLLNILIALMVRRPQHMLQV
FSLALVDVILLSCIFYAAGGSPSGIGNLIIVAVAIANILLRGRIGLLIAAVAAIGMIYLTFYLSLSRPEAANQYVQAGAL
GALCFAGALFVQGISRRLQLSETLAEQRAADVANLEALNALILQRMRTGILVLDSRHRVLLANQAAQAMLGHDGLAGRPL
GPHCPELLKRLEQWWDNPTLRPQSLQPVPDGTVLQPSFVQLQRGEQLDTLVFLEDISQIAQQAQQLKLASLGRLTAGIAH
EIRNPLGAISHAAQLLQESEELEGPDRRLAQIIQDHSRRMNLVIENVLQLSRRRQSEPQLLDLKYWLHRFASEFRSASPV
GQTLHLETNPGSIQTRMDPHQLTQVLTNLVQNGLRYSAQKHRQGQVWLKLFRDAESDLPILEVMDDGPGVAEEQLHHLFE
PFHTTENKGTGLGLYISRELCESNQARLDYRPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=79057 PotMrB4_RS05475 WP_074973001.1 1163641..1165236(-) (pilS) [Metapseudomonas otitidis strain MrB4]
GTGGCGAGCACTGAGTCGCCCTCCCTGGCGAGCAATCAGATCCGACGGATCTTCCGCCTTTACAACCTGTATCGCCTGCT
CATCGGCCTGGCGCTGGTCGGGCTGATTTCCAGTGAGCTGGACAGCGAGTTGCTGAACCTGGCCCGTGTCCAGCTGTTCC
GCAATGCCAGCTGGTTCTATCTACTGCTGAACATCCTCATTGCGCTGATGGTGCGCCGCCCCCAGCACATGCTGCAGGTG
TTCAGCCTGGCGCTGGTGGACGTCATCCTGCTGTCCTGCATCTTCTATGCAGCGGGCGGCTCGCCCAGTGGTATCGGCAA
CCTGATCATCGTTGCGGTGGCCATTGCCAACATCCTGCTGCGTGGGCGAATCGGGCTGCTGATCGCCGCCGTCGCCGCCA
TCGGGATGATCTACCTGACCTTCTACCTGAGCTTGTCGCGCCCGGAAGCGGCCAACCAGTACGTACAGGCCGGTGCGCTT
GGAGCCCTGTGCTTTGCAGGCGCCCTGTTCGTGCAGGGCATCAGCCGGCGCCTGCAGTTGAGCGAGACCCTGGCCGAGCA
GCGTGCTGCCGACGTGGCCAATCTGGAAGCGCTCAACGCGCTCATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTGG
ACAGCCGTCACCGGGTGTTGCTGGCCAACCAGGCTGCCCAGGCGATGCTGGGGCACGACGGGCTGGCGGGGCGCCCCCTC
GGGCCGCACTGCCCGGAGCTGCTCAAGCGGCTGGAGCAGTGGTGGGACAACCCGACCCTGCGCCCCCAGAGCCTGCAGCC
GGTACCCGATGGCACGGTGCTGCAGCCCAGCTTCGTCCAGCTGCAGCGCGGTGAGCAGCTGGATACGCTGGTCTTCCTCG
AAGATATCTCGCAGATCGCCCAGCAGGCGCAGCAGCTGAAGCTGGCCTCCCTCGGGCGGCTGACCGCCGGTATCGCCCAT
GAGATCCGCAATCCCCTGGGCGCCATCAGTCATGCCGCCCAGCTGTTGCAGGAGTCCGAAGAGCTGGAGGGGCCCGACCG
CCGCCTGGCGCAGATCATCCAGGATCATTCCCGGCGGATGAACCTCGTCATCGAGAACGTCCTGCAACTGTCGCGGCGCC
GCCAGTCGGAACCCCAGCTGCTGGATCTCAAGTACTGGCTGCACCGATTCGCCAGCGAGTTCCGCAGCGCCTCACCGGTG
GGCCAGACCCTTCACCTTGAGACCAATCCGGGGAGCATCCAGACCCGCATGGACCCCCACCAGCTCACCCAGGTGCTGAC
CAACCTGGTGCAGAACGGCCTGCGCTACAGCGCACAGAAGCATCGGCAGGGACAGGTCTGGCTGAAACTCTTCCGTGACG
CCGAGAGCGACCTGCCCATACTGGAAGTGATGGACGACGGCCCTGGCGTCGCCGAGGAGCAGCTCCATCACCTGTTCGAG
CCCTTCCACACCACGGAGAACAAGGGCACCGGGCTCGGCCTGTACATCTCCCGCGAGCTGTGCGAGAGCAACCAGGCCCG
CCTTGACTATCGACCCCGCGAAGGCGGCGGCAGCTGTTTCCGCATCACCTTCGCCCATCCGCGAAAACTGAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(418-524)

PAS domain (PAS_8)

(203-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I0USM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.686

98.493

0.667

Multiple sequence alignment