Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   PUW68_RS00225 Genome accession   NZ_CP118060
Coordinates   49097..50446 (-) Length   449 a.a.
NCBI ID   WP_275016267.1    Uniprot ID   -
Organism   Staphylococcus haemolyticus strain VSI35     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 44097..55446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW68_RS00190 (PUW68_00190) - 44148..44630 (-) 483 WP_275016260.1 hypothetical protein -
  PUW68_RS00195 (PUW68_00195) - 44669..45172 (-) 504 WP_275016261.1 hypothetical protein -
  PUW68_RS00200 (PUW68_00200) - 45294..45446 (-) 153 WP_275016262.1 hypothetical protein -
  PUW68_RS00205 (PUW68_00205) - 45715..46218 (-) 504 WP_275016263.1 DUF1643 domain-containing protein -
  PUW68_RS00210 (PUW68_00210) - 46233..46544 (-) 312 WP_275016264.1 DUF960 family protein -
  PUW68_RS00215 (PUW68_00215) - 46629..46979 (-) 351 WP_275016265.1 SAUGI family uracil-DNA glycosylase inhibitor -
  PUW68_RS00220 (PUW68_00220) ccrB 47452..49077 (-) 1626 WP_275016266.1 recombinase family protein Machinery gene
  PUW68_RS00225 (PUW68_00225) ccrA 49097..50446 (-) 1350 WP_275016267.1 recombinase family protein Machinery gene
  PUW68_RS00230 (PUW68_00230) - 50637..50912 (-) 276 WP_275016268.1 hypothetical protein -
  PUW68_RS00235 (PUW68_00235) - 50981..52768 (-) 1788 WP_275016269.1 DUF927 domain-containing protein -
  PUW68_RS00240 (PUW68_00240) - 52768..53037 (-) 270 WP_000875688.1 hypothetical protein -
  PUW68_RS00245 (PUW68_00245) - 53192..54751 (-) 1560 WP_275016270.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52143.07 Da        Isoelectric Point: 10.2713

>NTDB_id=789702 PUW68_RS00225 WP_275016267.1 49097..50446(-) (ccrA) [Staphylococcus haemolyticus strain VSI35]
MKQAIGYLRQSTLKQQSLAAQKQTIKALAKKHSIKQITFYSDKQSGRTDKRNGYQQVTELIQQGQCNVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKMITTHAPFGYHYH
NGTFTINKSEAPTVKAVFSYYLQGYGYKKIAQYLEANEKFINRKPYQVRNIIMNSNYCGRIINQYGQYDNMFPSIVSTNI
YEQAQAIRLQKQLKRTSSANQLKQKIKCPCCGSTLTNMTIRKKHHTLRYYVCPQNMNASRFICEFKGINAQTLEASVLST
CQDFFQNQHLYSKINHTIQQRLKKQKDIKAKSTVTQEQLIEKLAQGKIDAETFREQTQSFRQQSKPITSIHTHQIQKALQ
NVMQQRFTLNILYPYIDEIHITKNKTLTGIYFKNEPLNIVNQTTQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=789702 PUW68_RS00225 WP_275016267.1 49097..50446(-) (ccrA) [Staphylococcus haemolyticus strain VSI35]
ATGAAACAAGCAATAGGTTACTTACGTCAAAGTACACTCAAACAACAATCTCTAGCAGCACAGAAACAGACCATCAAAGC
ACTAGCCAAAAAACATAGTATTAAACAAATCACTTTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGAAATGGTT
ATCAACAAGTTACTGAACTGATTCAACAAGGACAATGTAACGTATTATGTTGCTATCGTCTGAATCGACTTCACCGTAAC
CTAAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAGTATCATGTTCATATCTTAAGCGTTCATGATGGCTACTTCGA
TATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCCGAACTAGAATCTGATAATATAGGCGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAAATGATTACGACCCATGCACCCTTTGGCTATCACTATCAT
AATGGGACATTCACAATAAACAAGTCAGAAGCACCAACAGTAAAAGCTGTATTCAGTTACTATCTTCAGGGTTATGGCTA
TAAAAAAATTGCACAATACTTAGAAGCTAATGAGAAATTTATCAATCGTAAACCCTATCAAGTACGTAATATTATCATGA
ACTCTAATTATTGTGGTCGAATCATTAATCAATACGGACAATATGACAACATGTTCCCTTCTATTGTTTCGACTAATATA
TATGAACAAGCTCAAGCGATACGTTTACAAAAGCAACTCAAACGGACATCATCAGCTAATCAACTCAAACAAAAAATCAA
ATGTCCGTGTTGTGGCTCAACACTCACAAATATGACCATTAGAAAAAAGCACCATACATTACGTTATTACGTCTGTCCTC
AAAATATGAATGCATCTCGTTTTATCTGTGAATTCAAAGGGATCAACGCACAAACATTAGAAGCGAGTGTCTTATCTACA
TGTCAAGACTTCTTTCAAAATCAACATCTGTATTCAAAAATCAACCATACTATTCAACAACGACTCAAAAAACAAAAAGA
CATAAAAGCTAAAAGTACAGTCACGCAAGAACAACTGATAGAAAAATTAGCCCAAGGCAAAATTGATGCAGAAACATTCA
GAGAACAAACGCAATCATTTCGACAACAGTCAAAACCTATAACATCAATCCATACGCATCAAATTCAAAAGGCTTTACAA
AATGTCATGCAACAACGTTTCACATTAAACATATTGTACCCTTACATTGATGAAATTCATATTACCAAAAATAAAACACT
TACGGGTATCTATTTCAAAAACGAGCCCTTAAACATCGTCAATCAAACAACACAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

85.969

100

0.86
  ccrA Staphylococcus aureus N315

76.392

100

0.764