Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   PUW68_RS00220 Genome accession   NZ_CP118060
Coordinates   47452..49077 (-) Length   541 a.a.
NCBI ID   WP_275016266.1    Uniprot ID   -
Organism   Staphylococcus haemolyticus strain VSI35     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 42452..54077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW68_RS00180 (PUW68_00180) - 42951..43175 (-) 225 Protein_33 recombinase family protein -
  PUW68_RS00185 (PUW68_00185) - 43285..43794 (+) 510 WP_275016259.1 hypothetical protein -
  PUW68_RS00190 (PUW68_00190) - 44148..44630 (-) 483 WP_275016260.1 hypothetical protein -
  PUW68_RS00195 (PUW68_00195) - 44669..45172 (-) 504 WP_275016261.1 hypothetical protein -
  PUW68_RS00200 (PUW68_00200) - 45294..45446 (-) 153 WP_275016262.1 hypothetical protein -
  PUW68_RS00205 (PUW68_00205) - 45715..46218 (-) 504 WP_275016263.1 DUF1643 domain-containing protein -
  PUW68_RS00210 (PUW68_00210) - 46233..46544 (-) 312 WP_275016264.1 DUF960 family protein -
  PUW68_RS00215 (PUW68_00215) - 46629..46979 (-) 351 WP_275016265.1 SAUGI family uracil-DNA glycosylase inhibitor -
  PUW68_RS00220 (PUW68_00220) ccrB 47452..49077 (-) 1626 WP_275016266.1 recombinase family protein Machinery gene
  PUW68_RS00225 (PUW68_00225) ccrA 49097..50446 (-) 1350 WP_275016267.1 recombinase family protein Machinery gene
  PUW68_RS00230 (PUW68_00230) - 50637..50912 (-) 276 WP_275016268.1 hypothetical protein -
  PUW68_RS00235 (PUW68_00235) - 50981..52768 (-) 1788 WP_275016269.1 DUF927 domain-containing protein -
  PUW68_RS00240 (PUW68_00240) - 52768..53037 (-) 270 WP_000875688.1 hypothetical protein -

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 62441.36 Da        Isoelectric Point: 9.6019

>NTDB_id=789701 PUW68_RS00220 WP_275016266.1 47452..49077(-) (ccrB) [Staphylococcus haemolyticus strain VSI35]
MNEMKKKRIGAYIRVSTERQVEGYSIEGQITQIEQYCQFNSYELVDIYADRGISGKSMNRPELQRMLKDAKNGKLDCVMV
YKTNRLARNTSDLLTIVEELHRQNVEFFSLSERMEVKNSTGKLMLQILASFSEFERNTILENIYTGQHQRALEGYYQGNL
PLGYNNIPDNKKELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTSKGNPFSISAVTYILSNPFYIGKIQFAKY
KDWNDKRRKGLNDKPVIAEGKHAPIIGKDLWDKVQARKKQVSEKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRAEVIEKYVMDQILEIVKSDKVLKQVVERVNQDSQIDMAALNHDIAYKQQQFDE
INTKLKNLIQTIEDNPDLTSALKPTIHQYETQLNDITNQMNQLKHQQNQEKPSYDTKQIAALLQRIFQNIESMDKSQLKA
FYLTVIDRIDIRKDENHKKQFYVTLKLNNEIIKQLFNDTPLDEVLLSTSSLFLPQTLYFQI

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=789701 PUW68_RS00220 WP_275016266.1 47452..49077(-) (ccrB) [Staphylococcus haemolyticus strain VSI35]
ATGAATGAAATGAAGAAGAAGAGAATCGGAGCGTACATTCGCGTGTCCACAGAGAGACAAGTAGAGGGTTATAGTATTGA
GGGACAAATTACACAAATAGAGCAATATTGCCAATTTAACAGCTATGAACTTGTTGATATATATGCAGATCGGGGTATAT
CAGGAAAATCTATGAACCGTCCTGAATTACAGCGCATGTTAAAAGATGCTAAAAATGGAAAATTAGACTGTGTTATGGTT
TATAAGACAAATCGTTTGGCACGTAATACTTCCGATTTACTTACAATCGTCGAAGAACTTCATCGCCAAAATGTTGAATT
TTTTAGCTTGTCTGAACGCATGGAAGTCAAAAATTCAACAGGCAAGTTAATGCTCCAGATACTTGCAAGTTTTTCTGAAT
TTGAAAGAAATACAATTTTAGAGAATATTTACACCGGGCAACATCAAAGAGCTTTAGAGGGCTATTATCAAGGCAATCTT
CCATTAGGATATAATAACATACCTGATAATAAAAAAGAATTAATGATTAATCAACATGAAGCTAACATCGTTAAATATAT
CTTTGAATCTTATGCCAAAGGTCATGGTTATCGTAAAATAGCCAATGCACTCAATCATAAAGGTTATGTCACTAGTAAGG
GTAATCCATTTAGTATTTCAGCTGTTACTTATATTCTCTCAAATCCATTCTATATTGGTAAAATTCAATTCGCAAAATAC
AAAGATTGGAATGATAAACGACGTAAAGGCTTGAATGATAAGCCAGTAATCGCTGAAGGTAAGCATGCCCCTATTATTGG
CAAAGACTTATGGGATAAAGTACAAGCACGTAAAAAACAAGTAAGTGAAAAACCACAAGTGCATGGTAAAGGAACCAATC
TATTAACGGGTATTATTCACTGCCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACAAACACATTGAAAGATGGT
ACTAAGAAGCGAATACGCTATTATTCTTGTAGTAATTTTCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGTGTTAG
AGCTGAGGTGATTGAGAAATACGTTATGGATCAAATACTTGAAATTGTCAAAAGCGATAAAGTTCTCAAACAAGTTGTCG
AACGTGTTAATCAAGATAGTCAAATTGATATGGCTGCACTTAACCATGATATCGCATATAAGCAACAACAATTTGATGAA
ATAAACACTAAACTAAAAAATCTAATTCAAACCATCGAAGACAATCCAGACTTAACATCTGCACTCAAACCAACCATTCA
TCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAGCACCAACAAAATCAAGAGAAACCATCTT
ATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCCATGGATAAATCACAGCTCAAAGCT
TTTTACCTAACGGTTATTGACCGTATTGACATTCGTAAAGATGAGAATCATAAAAAGCAATTCTACGTTACACTAAAACT
TAATAATGAAATTATTAAACAACTTTTCAATGATACCCCACTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTTTGC
CTCAAACACTCTACTTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-154)

Recombinase (Recombinase)

(179-279)

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

79.889

100

0.8