Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PUW46_RS07595 Genome accession   NZ_CP118044
Coordinates   1588511..1589197 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain VSI56     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1583511..1594197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW46_RS07575 (PUW46_07575) rpsT 1584425..1584676 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  PUW46_RS07580 (PUW46_07580) holA 1584721..1585695 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  PUW46_RS07585 (PUW46_07585) comEC 1585752..1587953 (-) 2202 WP_025173822.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUW46_RS07590 (PUW46_07590) - 1587958..1588419 (-) 462 WP_000439693.1 ComE operon protein 2 -
  PUW46_RS07595 (PUW46_07595) comEA 1588511..1589197 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  PUW46_RS07600 (PUW46_07600) - 1589237..1589953 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  PUW46_RS07605 (PUW46_07605) rsfS 1589956..1590309 (-) 354 WP_001088022.1 ribosome silencing factor -
  PUW46_RS07610 (PUW46_07610) yqeK 1590310..1590894 (-) 585 WP_001019323.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PUW46_RS07615 (PUW46_07615) nadD 1590884..1591453 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  PUW46_RS07620 (PUW46_07620) yhbY 1591456..1591746 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  PUW46_RS07625 (PUW46_07625) aroE 1591750..1592556 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  PUW46_RS07630 (PUW46_07630) yqeH 1592570..1593670 (-) 1101 WP_001280138.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=789421 PUW46_RS07595 WP_001792568.1 1588511..1589197(-) (comEA) [Staphylococcus aureus strain VSI56]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=789421 PUW46_RS07595 WP_001792568.1 1588511..1589197(-) (comEA) [Staphylococcus aureus strain VSI56]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969