Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpX   Type   Regulator
Locus tag   PUW70_RS04320 Genome accession   NZ_CP118029
Coordinates   845346..846578 (+) Length   410 a.a.
NCBI ID   WP_024052280.1    Uniprot ID   A0A412PMJ3
Organism   Streptococcus anginosus strain VSI68     
Function   require for competence development (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 838239..854440 845346..846578 within 0


Gene organization within MGE regions


Location: 838239..854440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW70_RS04265 (PUW70_04265) - 838452..838622 (-) 171 Protein_801 helix-turn-helix transcriptional regulator -
  PUW70_RS04270 (PUW70_04270) - 839127..839549 (+) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  PUW70_RS04275 (PUW70_04275) - 839546..839776 (+) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  PUW70_RS04280 (PUW70_04280) - 840002..840253 (-) 252 WP_001845478.1 hypothetical protein -
  PUW70_RS04285 (PUW70_04285) - 840237..840440 (+) 204 WP_000814511.1 excisionase -
  PUW70_RS04290 (PUW70_04290) - 840522..841739 (+) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -
  PUW70_RS04295 (PUW70_04295) - 842014..842280 (+) 267 WP_024052284.1 chorismate mutase -
  PUW70_RS04300 (PUW70_04300) - 842295..843512 (+) 1218 WP_024052283.1 voltage-gated chloride channel family protein -
  PUW70_RS04305 (PUW70_04305) - 843598..844437 (+) 840 WP_024052282.1 thymidylate synthase -
  PUW70_RS04310 (PUW70_04310) - 844622..845134 (+) 513 WP_024052281.1 dihydrofolate reductase -
  PUW70_RS04315 (PUW70_04315) - 845134..845307 (+) 174 WP_003024903.1 hypothetical protein -
  PUW70_RS04320 (PUW70_04320) clpX 845346..846578 (+) 1233 WP_024052280.1 ATP-dependent Clp protease ATP-binding subunit ClpX Regulator
  PUW70_RS04325 (PUW70_04325) yihA 846590..847177 (+) 588 WP_024052279.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  PUW70_RS04330 (PUW70_04330) - 847358..848065 (+) 708 WP_003024892.1 glucosaminidase domain-containing protein -
  PUW70_RS04335 (PUW70_04335) - 848248..849993 (+) 1746 WP_070815110.1 ABC transporter ATP-binding protein -
  PUW70_RS04340 (PUW70_04340) - 849974..851722 (+) 1749 WP_070815111.1 ABC transporter ATP-binding protein -
  PUW70_RS04345 (PUW70_04345) - 851743..853278 (-) 1536 WP_274996364.1 ABC-F family ATP-binding cassette domain-containing protein -
  PUW70_RS04350 (PUW70_04350) - 853700..854440 (-) 741 WP_070241651.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45718.24 Da        Isoelectric Point: 4.4242

>NTDB_id=789248 PUW70_RS04320 WP_024052280.1 845346..846578(+) (clpX) [Streptococcus anginosus strain VSI68]
MPTNRTNEMMVYCSFCGKSQEEVQKIIAGNNAFICNECVELAQEIIREELAEEVLADLSEVPKPQELLNILNHYVIGQDR
AKRALAVAVYNHYKRINFHDNREEEDDVELQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
ILLKLLQAADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGTVASVPPQGGRKHPQQEMIQVDT
KNILFIVGGAFDSIEEIVKQRLGEKIIGFGQNNKAIDETGSYMQEIISEDIQKFGLIPELIGRLPVFAALEPLTVDDLVR
ILREPKNALVKQYQTLLSYDDVKLEFDDDALQEIANKAIERKTGARGLRSIIEETMMDVMFEVPSQENVKLVRITKEAVD
GTDKPILETA

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=789248 PUW70_RS04320 WP_024052280.1 845346..846578(+) (clpX) [Streptococcus anginosus strain VSI68]
ATGCCTACAAATCGTACGAATGAAATGATGGTTTATTGCTCTTTTTGTGGTAAAAGCCAAGAAGAAGTCCAAAAAATTAT
CGCAGGAAATAATGCCTTTATTTGTAATGAATGCGTGGAATTAGCGCAAGAAATCATCAGAGAGGAACTAGCAGAAGAAG
TACTAGCGGACTTGTCAGAAGTGCCAAAACCGCAAGAACTACTCAATATTTTGAATCATTATGTGATTGGGCAAGATCGT
GCTAAGCGAGCATTGGCTGTTGCTGTATACAATCACTATAAACGTATCAATTTTCACGATAATCGTGAAGAAGAGGATGA
TGTCGAATTACAAAAATCCAATATTCTTATGATTGGGCCAACTGGTTCTGGTAAGACTTTTCTTGCTCAAACCTTGGCGA
AAAGTCTGAATGTTCCTTTTGCGATTGCAGATGCGACAGCTTTGACAGAAGCTGGGTATGTCGGAGAAGATGTTGAAAAT
ATTCTCTTGAAACTCTTGCAAGCAGCAGATTTCAATATTGAGCGTGCAGAACGTGGTATCATCTATGTAGATGAGATTGA
CAAAATTGCGAAGAAAAGCGAAAACGTGTCTATTACTCGCGATGTGTCTGGTGAAGGCGTTCAGCAAGCGCTCCTCAAGA
TTATTGAAGGAACTGTAGCCAGCGTACCTCCGCAAGGCGGTCGCAAGCACCCTCAACAAGAAATGATTCAAGTAGATACC
AAGAATATTCTCTTTATCGTTGGTGGTGCATTTGACAGTATTGAAGAAATTGTCAAACAACGTCTAGGCGAAAAAATTAT
CGGTTTTGGTCAAAATAATAAAGCCATTGATGAAACTGGTTCTTATATGCAAGAAATCATCTCTGAAGATATTCAAAAAT
TTGGTTTGATTCCGGAATTGATTGGTCGTTTGCCTGTGTTTGCAGCGCTTGAGCCTTTGACAGTTGATGATTTGGTGCGA
ATCCTGCGCGAACCAAAGAATGCTTTAGTGAAGCAATATCAGACTCTCCTTTCTTATGACGATGTTAAACTAGAGTTTGA
TGATGATGCTCTTCAGGAAATTGCTAACAAGGCCATTGAACGCAAGACAGGTGCGCGTGGATTACGTTCAATCATTGAGG
AAACTATGATGGATGTTATGTTTGAAGTTCCAAGTCAAGAAAATGTGAAGCTGGTACGGATTACCAAGGAAGCAGTTGAT
GGAACGGATAAGCCGATTTTAGAAACAGCATAA

Domains

Predicted by InterproScan.

ClpX C4-type zinc finger (zf-C4_ClpX)

(12-48)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(313-390)

AAA domain (Cdc48 subfamily) (AAA_2)

(111-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PMJ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpX Streptococcus mutans UA159

86.341

100

0.863
  clpX Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

57.96

98.049

0.568