Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PT671_RS18075 Genome accession   NZ_CP118021
Coordinates   3606382..3607452 (+) Length   356 a.a.
NCBI ID   WP_003226312.1    Uniprot ID   -
Organism   Bacillus spizizenii strain B-354     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3601382..3612452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS18045 mgsR 3601777..3602154 (+) 378 WP_003226302.1 transcriptional regulator MgsR -
  PT671_RS18055 - 3602380..3603219 (+) 840 WP_003226304.1 STAS domain-containing protein -
  PT671_RS18060 - 3603271..3604599 (-) 1329 WP_044428668.1 hemolysin family protein -
  PT671_RS18065 - 3604743..3605696 (+) 954 WP_003226308.1 magnesium transporter CorA family protein -
  PT671_RS18070 - 3605759..3606169 (+) 411 WP_032727412.1 CBS domain-containing protein -
  PT671_RS18075 comGA 3606382..3607452 (+) 1071 WP_003226312.1 competence protein ComGA Machinery gene
  PT671_RS18080 comGB 3607439..3608476 (+) 1038 WP_003226315.1 competence type IV pilus assembly protein ComGB Machinery gene
  PT671_RS18085 comGC 3608490..3608786 (+) 297 WP_003226319.1 comG operon protein ComGC Machinery gene
  PT671_RS18090 comGD 3608776..3609207 (+) 432 WP_003226321.1 competence type IV pilus minor pilin ComGD Machinery gene
  PT671_RS18095 comGE 3609191..3609538 (+) 348 WP_003226323.1 competence type IV pilus minor pilin ComGE Machinery gene
  PT671_RS18100 comGF 3609564..3609947 (+) 384 WP_032727308.1 competence type IV pilus minor pilin ComGF Machinery gene
  PT671_RS18105 comGG 3609948..3610322 (+) 375 WP_003226328.1 competence type IV pilus minor pilin ComGG Machinery gene
  PT671_RS18110 - 3610394..3610573 (+) 180 WP_003226330.1 YqzE family protein -
  PT671_RS18115 - 3610615..3610941 (-) 327 WP_079996407.1 YqzG/YhdC family protein -
  PT671_RS18120 tapA 3611210..3611971 (+) 762 WP_003226335.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40489.75 Da        Isoelectric Point: 9.0214

>NTDB_id=789184 PT671_RS18075 WP_003226312.1 3606382..3607452(+) (comGA) [Bacillus spizizenii strain B-354]
MDSIEKISKTLIEEAYITKASDIHIVPRERDAIIHFRVDHALLKKRHMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLEEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQLRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRHIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=789184 PT671_RS18075 WP_003226312.1 3606382..3607452(+) (comGA) [Bacillus spizizenii strain B-354]
TTGGATTCAATAGAAAAGATAAGCAAAACCTTGATTGAAGAGGCATATATAACAAAGGCTTCTGACATTCACATTGTACC
GAGGGAGCGGGACGCCATCATTCATTTTCGGGTTGATCATGCCTTGCTGAAAAAAAGGCACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTCTCAGCGATGGACATAGGGGAAAGGAGGAAGCCGCAAAATGGTTCGCTTACA
TTAAAGCTAGAGGAGGGAAATGTTCATTTAAGAATGTCAACGCTCCCCACAATTAATGAAGAAAGCCTTGTGATCAGAGT
GATGCCTCAATATAATATCCCTTCGATTGATAAGCTGTCATTATTTCCGAAGACAGGAGCCACTTTACTCTCATTTTTAA
AACATTCCCACGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACCACCACATTATACTCTCTTGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACACTGGAGGACCCTGTTGAAACAAGGGACGAGGATGTTCTTCAGGTGCA
GGTGAATGAAAAAGCCGGTGTGACATATTCCGCAGGGCTGAAGGCTATTTTGCGCCATGACCCGGATATGATTATCTTAG
GTGAGATCAGAGACGCCGAAACAGCTAAAATCGCGGTACGGGCAGCGATGACGGGGCATCTGGTGCTGACGAGCCTTCAT
ACGAGAGATGCGAAGGGCGCGATTTACAGGCTGCTTGAATTCGGCATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTTAC
GAAACACCAGGAGAGCAAGCGTTTATGAGCTCCTTTACGGAAAAAATCTTCAGCAATGTATTCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTACGTCACATTATCCGAAAAGGAATTGCACTCGGCTATTTGACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

97.472

100

0.975