Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PT671_RS17625 Genome accession   NZ_CP118021
Coordinates   3526094..3526711 (+) Length   205 a.a.
NCBI ID   WP_003226145.1    Uniprot ID   -
Organism   Bacillus spizizenii strain B-354     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3521094..3531711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS17590 aroE 3521752..3522594 (+) 843 WP_003226130.1 shikimate dehydrogenase -
  PT671_RS17595 yhbY 3522588..3522878 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PT671_RS17600 - 3522890..3523459 (+) 570 WP_003226135.1 nicotinate-nucleotide adenylyltransferase -
  PT671_RS17605 yqeK 3523449..3524009 (+) 561 WP_003226137.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PT671_RS17610 rsfS 3524027..3524383 (+) 357 WP_003226139.1 ribosome silencing factor -
  PT671_RS17615 - 3524380..3525123 (+) 744 WP_003226141.1 class I SAM-dependent DNA methyltransferase -
  PT671_RS17620 comER 3525189..3526010 (-) 822 WP_003226143.1 late competence protein ComER -
  PT671_RS17625 comEA 3526094..3526711 (+) 618 WP_003226145.1 helix-hairpin-helix domain-containing protein Machinery gene
  PT671_RS17630 - 3526778..3527347 (+) 570 WP_003226147.1 ComE operon protein 2 -
  PT671_RS17635 comEC 3527351..3529681 (+) 2331 WP_003226149.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PT671_RS17640 - 3529722..3529856 (-) 135 WP_003226152.1 YqzM family protein -
  PT671_RS17645 - 3529897..3530046 (+) 150 WP_136975859.1 hypothetical protein -
  PT671_RS17650 holA 3530086..3531129 (+) 1044 WP_003226154.1 DNA polymerase III subunit delta -
  PT671_RS17655 rpsT 3531144..3531410 (-) 267 WP_003226155.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21919.78 Da        Isoelectric Point: 5.1470

>NTDB_id=789180 PT671_RS17625 WP_003226145.1 3526094..3526711(+) (comEA) [Bacillus spizizenii strain B-354]
MNWLNHHKKAIILGASAAVFIAIIFFLASGKNKESMKQTVSTETENTEVKQEAVKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAGLLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=789180 PT671_RS17625 WP_003226145.1 3526094..3526711(+) (comEA) [Bacillus spizizenii strain B-354]
ATGAATTGGTTGAATCATCATAAGAAAGCAATTATTTTAGGGGCCTCTGCGGCTGTTTTCATAGCGATCATTTTCTTCCT
GGCCAGCGGGAAAAACAAAGAGTCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAG
TCAAAGATGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCACCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCCCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGG
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGAAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATTACGAAGGT
TTCAGGGATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

89.268

100

0.893
  comEA Latilactobacillus sakei subsp. sakei 23K

31.646

100

0.366
  comEA Staphylococcus aureus MW2

37.688

97.073

0.366