Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PT671_RS09280 Genome accession   NZ_CP118021
Coordinates   1888725..1890101 (-) Length   458 a.a.
NCBI ID   WP_003225734.1    Uniprot ID   E0TYL6
Organism   Bacillus spizizenii strain B-354     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1883725..1895101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS09260 ispF 1885243..1885719 (-) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PT671_RS09265 ispD 1885712..1886410 (-) 699 WP_003225740.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PT671_RS09270 - 1886425..1887525 (-) 1101 WP_003225738.1 PIN/TRAM domain-containing protein -
  PT671_RS09275 disA 1887639..1888721 (-) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  PT671_RS09280 radA 1888725..1890101 (-) 1377 WP_003225734.1 DNA repair protein RadA Machinery gene
  PT671_RS09285 clpC 1890193..1892625 (-) 2433 WP_003225731.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  PT671_RS09290 - 1892622..1893713 (-) 1092 WP_003225728.1 protein arginine kinase -
  PT671_RS09295 mcsA 1893713..1894270 (-) 558 WP_003225726.1 protein-arginine kinase activator protein McsA -
  PT671_RS09300 ctsR 1894284..1894748 (-) 465 WP_003225724.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49442.88 Da        Isoelectric Point: 7.6840

>NTDB_id=789149 PT671_RS09280 WP_003225734.1 1888725..1890101(-) (radA) [Bacillus spizizenii strain B-354]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINSPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFTRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=789149 PT671_RS09280 WP_003225734.1 1888725..1890101(-) (radA) [Bacillus spizizenii strain B-354]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACTATGGTGGAAGAAATGATAAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCAGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATTGAAACATCAGAAGAACCCCGGGTTAAAACCCAGCTTGGCGAGTTT
AACAGAGTGCTCGGCGGGGGCGTCGTCAAAGGCTCACTCGTTTTAATCGGCGGCGATCCTGGTATCGGAAAGTCAACGTT
ATTACTGCAGGTTTCCGCTCAATTATCAGATTCATCGAACAGTGTGCTGTACATTTCGGGAGAAGAATCGGTAAAGCAAA
CGAAACTGAGAGCAGATCGTCTTGGCATTAATAGTCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTAGACTCTATTCAAACGGTTTACCAAAGCGACATTACATCGGC
TCCGGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAGGAAGGGTCGATTGCAGGGCCGAGACTGTTGGAGCATATGGTCGACACTGTTTTATATTTT
GAAGGGGAACGTCACCATACGTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGTTCTACAAATGAAATGGGCATTTT
TGAAATGCGCGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAAGAACGTTCAGCGGGATCCGCAG
GCTCAAGCATAACTGCTTCTATGGAGGGCACAAGACCGATTCTCGTTGAAATCCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGTATGGCAACAGGAATAGACCATAACAGGGTTTCGCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTAGCCGGCGGCGTAAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGTATCGCGTCCAGCTTTAGAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGCCGGGTTTCAAGAATTGAACAACGTGTGAAAGAAGCGGCTAAGCTTGGTTTTACACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0TYL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.908

100

0.989
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627