Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PUT72_RS13700 Genome accession   NZ_CP117994
Coordinates   2913075..2913668 (-) Length   197 a.a.
NCBI ID   WP_005381322.1    Uniprot ID   A0AAW9BKV9
Organism   Vibrio alginolyticus strain 20220413_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2908075..2918668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT72_RS13680 (PUT72_13680) aroB 2908960..2910060 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  PUT72_RS13685 (PUT72_13685) aroK 2910093..2910611 (-) 519 WP_005381319.1 shikimate kinase AroK -
  PUT72_RS13690 (PUT72_13690) pilQ 2910799..2912544 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  PUT72_RS13695 (PUT72_13695) pilP 2912570..2913085 (-) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  PUT72_RS13700 (PUT72_13700) pilO 2913075..2913668 (-) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUT72_RS13705 (PUT72_13705) pilN 2913661..2914236 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  PUT72_RS13710 (PUT72_13710) pilM 2914220..2915236 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  PUT72_RS13715 (PUT72_13715) - 2915381..2917942 (+) 2562 WP_085570127.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23015.53 Da        Isoelectric Point: 5.0885

>NTDB_id=789044 PUT72_RS13700 WP_005381322.1 2913075..2913668(-) (pilO) [Vibrio alginolyticus strain 20220413_2]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=789044 PUT72_RS13700 WP_005381322.1 2913075..2913668(-) (pilO) [Vibrio alginolyticus strain 20220413_2]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.368

96.447

0.843
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685