Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   PUN35_RS02325 Genome accession   NZ_CP117986
Coordinates   424860..426590 (+) Length   576 a.a.
NCBI ID   WP_014480229.1    Uniprot ID   -
Organism   Bacillus sp. N3378-2at     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 419860..431590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS02300 - 420224..420478 (+) 255 WP_003226409.1 exodeoxyribonuclease VII small subunit -
  PUN35_RS02305 - 420468..421358 (+) 891 WP_003230262.1 polyprenyl synthetase family protein -
  PUN35_RS02310 dxs 421533..423434 (+) 1902 WP_014480232.1 1-deoxy-D-xylulose-5-phosphate synthase -
  PUN35_RS02315 - 423431..424276 (+) 846 WP_031600677.1 TlyA family RNA methyltransferase -
  PUN35_RS02320 argR 424374..424823 (+) 450 WP_003230265.1 arginine repressor ArgR -
  PUN35_RS02325 recN 424860..426590 (+) 1731 WP_014480229.1 DNA repair protein RecN Machinery gene
  PUN35_RS02330 spoIVB 426765..428045 (+) 1281 WP_014480228.1 SpoIVB peptidase -
  PUN35_RS02335 spo0A 428321..429124 (+) 804 WP_003226427.1 sporulation transcription factor Spo0A Regulator
  PUN35_RS02340 - 429589..430707 (-) 1119 WP_017696437.1 NADH-dependent flavin oxidoreductase -
  PUN35_RS02345 - 430780..430932 (-) 153 WP_014480226.1 hypothetical protein -
  PUN35_RS02350 - 431240..431536 (+) 297 WP_014480225.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 64541.40 Da        Isoelectric Point: 4.7429

>NTDB_id=788882 PUN35_RS02325 WP_014480229.1 424860..426590(+) (recN) [Bacillus sp. N3378-2at]
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEAKAELEGLFLLESGHPVLGVC
AEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLREIGRLLLDIHGQHDNQLLMEDENHLELLDKFAGAEVESALK
TYQEGYQRYMKLLKKLKQLSESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLNYIETRLNEIKQLKRKYGATV
EDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAVEAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKV
RTASRNEEAPLVNGQPVQLTEQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQEKVAEIGRMIAGVEVTDLTKR
HAKELLKQADQVKTTG

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=788882 PUN35_RS02325 WP_014480229.1 424860..426590(+) (recN) [Bacillus sp. N3378-2at]
TTGTTAGCTGAACTATCGATTAAAAACTTTGCCATTATTGAGGAACTGACGGTTTCTTTTGAACGCGGGCTTACGGTTTT
AACAGGAGAAACCGGAGCCGGCAAATCCATCATCATAGATGCCATTTCTCTTTTAGTCGGAGGCCGCGGATCATCTGAAT
TTGTTAGATATGGGGAAGCAAAAGCCGAACTTGAAGGGCTGTTTCTGTTGGAGAGCGGCCATCCTGTTTTAGGCGTATGC
GCTGAGCAGGGAATCGACGTTTCAGACGAAATGATCGTCATGAGAAGGGATATCAGCACGAGCGGGAAAAGCGTTTGCCG
TGTCAATGGCAAGCTTGTCACGATTGCTTCGCTAAGAGAGATCGGCAGGCTTTTATTAGATATTCACGGACAGCACGATA
ACCAGCTGTTAATGGAAGACGAAAATCATCTCGAGCTTTTGGACAAGTTTGCCGGGGCCGAAGTGGAAAGCGCGCTCAAA
ACGTATCAGGAAGGATACCAGCGGTATATGAAGCTGCTCAAAAAGCTTAAGCAGCTTTCTGAAAGCGAACAGGAAATGGC
CCATCGTCTTGATCTTATTCAATTTCAGCTCGAAGAAATCGAATCGGCAAAGCTTGAGCTTAACGAAGATGAACAGCTCC
AAGAAGAGCGCCAGCAAATTTCAAACTTTGAGAAGATATATGAGTCGCTGCAAAATGCATATAACGCGCTTCGCAGTGAG
CAGGGCGGATTGGACTGGGTTGGAATGGCATCAGCGCAGCTTGAAGATATATCTGATATAAACGAACCGCTGAAAAAAAT
GTCTGAAAGTGTTTCGAACTCCTACTATTTGCTTGAGGATGCCACATTTCAGATGAGAAATATGCTGGATGAGCTTGAAT
TTGATCCGGAACGCCTGAACTATATTGAGACGCGACTTAATGAAATCAAACAGCTGAAACGAAAATACGGTGCCACTGTT
GAAGACATTTTGGAATATGCTTCTAAAATTGAAGAAGAGATTGACCAAATTGAAAACAGGGACAGCCACCTTCAAAGCTT
GAAAAAGGAGCTTGACTCCGTCGGCAAAGATGTCGCAGTGGAAGCGGCAAATGTGTCACAAATCCGAAAAACATGGGCGA
AAAAGCTGGCTGATGAGATTCATCGGGAATTAAAAAGCCTGTACATGGAAAAATCAACTTTTGATACGGAATTTAAGGTC
AGAACAGCGTCACGCAATGAAGAGGCTCCGCTTGTAAACGGCCAGCCCGTTCAGCTGACTGAACAAGGGATTGACCTGGT
GAAGTTTTTAATTTCGACCAATACAGGCGAGCCGCTGAAATCCCTTTCGAAAGTCGCTTCAGGTGGAGAGCTGTCACGGG
TGATGCTTGCCATAAAAAGCATTTTTTCTTCACAACAGGATGTGACATCGATCATTTTTGATGAGGTTGATACCGGTGTA
AGCGGCAGGGTCGCACAGGCAATCGCAGAGAAGATCCATAAGGTGTCAATAGGATCACAAGTGCTGTGCATTACACACCT
GCCTCAGGTGGCTGCCATGGCCGATACGCATCTTTATATTGCGAAGGAGCTAAAAGACGGCAGAACGACGACACGTGTCA
AGCCGCTCTCTAAGCAGGAAAAGGTAGCGGAAATCGGGCGCATGATTGCAGGTGTCGAAGTAACCGACTTAACGAAACGC
CATGCGAAAGAACTTTTAAAACAAGCGGATCAAGTCAAAACAACTGGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_23)

(4-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

99.653

100

0.997