Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PSH71_RS25885 Genome accession   NZ_CP117457
Coordinates   5805910..5807256 (+) Length   448 a.a.
NCBI ID   WP_003205703.1    Uniprot ID   F2KLF1
Organism   Pseudomonas brassicacearum strain FP1843     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5800910..5812256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH71_RS25860 (PSH71_25855) pgeF 5801123..5801848 (-) 726 WP_003205694.1 peptidoglycan editing factor PgeF -
  PSH71_RS25865 (PSH71_25860) rluD 5801845..5802807 (-) 963 WP_003205696.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSH71_RS25870 (PSH71_25865) - 5802958..5803974 (+) 1017 WP_003205698.1 outer membrane protein assembly factor BamD -
  PSH71_RS25875 (PSH71_25870) - 5804101..5804334 (+) 234 WP_013694237.1 PP0621 family protein -
  PSH71_RS25880 (PSH71_25875) pilS 5804324..5805913 (+) 1590 WP_003205701.1 nitrogen regulation protein NR(II) Regulator
  PSH71_RS25885 (PSH71_25880) pilR 5805910..5807256 (+) 1347 WP_003205703.1 sigma-54 dependent transcriptional regulator Regulator
  PSH71_RS25890 (PSH71_25885) - 5807348..5807713 (-) 366 WP_003205705.1 DUF6124 family protein -
  PSH71_RS25895 (PSH71_25890) - 5808026..5808214 (+) 189 WP_155252615.1 nucleoid-structuring protein H-NS -
  PSH71_RS25900 (PSH71_25895) thiO 5808946..5810046 (-) 1101 WP_003205709.1 glycine oxidase ThiO -
  PSH71_RS25905 (PSH71_25900) - 5810102..5810503 (-) 402 WP_003205711.1 type IV pilin protein -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 50084.49 Da        Isoelectric Point: 6.2020

>NTDB_id=785623 PSH71_RS25885 WP_003205703.1 5805910..5807256(+) (pilR) [Pseudomonas brassicacearum strain FP1843]
MNNRSRQRILIVDDEPDIRELLDITLGRMKLDTRSAKNLAEAQALLAGEAFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAIDALKAGAFDFLTKPVDLGRLRELVTSALRLPPVATPGTTLERSLLGNSPPMLSVRKQIGKLARSQA
PVYISGESGCGKELVARLIHEQGPRANQRFVPVNCGAIPTELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVA
DLPLAMQVKLLRAIQEKAVRAVGGQQEEVVDVRILCATHKDLDGEVAAGRFRQDLYYRLNVIELRVPPLRERREDIELLA
NHMLQRLAANTGSPPVRLHPQALDALKNYRFPGNVRELENMLERAYTLCEQQQIEADDLRLAEGNSTTEAGNPDLMQVDN
LEDYLEDVERKLILQALEETRWNRTAAAQRLKLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=785623 PSH71_RS25885 WP_003205703.1 5805910..5807256(+) (pilR) [Pseudomonas brassicacearum strain FP1843]
TTGAATAATCGCTCACGGCAACGAATCCTGATCGTCGATGACGAACCGGACATCCGCGAACTCCTGGACATCACCCTGGG
CCGGATGAAACTCGACACCCGCAGCGCCAAGAACCTTGCCGAGGCCCAAGCCCTGCTGGCGGGGGAAGCCTTCGACCTGT
GCCTGACCGACATGCGCCTGCCCGATGGCACCGGCCTGGAACTGGTGCAGCACATCCAGCAACGCTACCCCCAACTGCCC
GTGGCGATGATCACCGCCTATGGCAGCCTGGAAACCGCCATCGACGCCCTCAAGGCCGGCGCCTTCGACTTCCTGACCAA
ACCGGTCGACCTGGGTCGGCTGCGAGAGCTGGTCACCAGTGCCCTGCGCCTGCCGCCGGTCGCCACGCCGGGCACGACCC
TCGAGCGCAGCCTGCTGGGTAACTCGCCACCGATGCTCAGCGTGCGCAAGCAGATCGGGAAACTCGCCCGCAGCCAGGCA
CCGGTGTACATCAGCGGGGAATCGGGCTGCGGCAAGGAACTGGTCGCCCGGCTGATCCACGAACAGGGCCCTCGTGCCAA
CCAGCGCTTCGTACCGGTCAACTGTGGGGCGATTCCCACGGAGCTGATGGAAAGCGAGTTTTTCGGCCATCGAAAAGGCA
GCTTCAGCGGCGCCATCGAGGACAAGCCCGGGCTGTTCCAGGCCGCCCATGGCGGGACCCTGTTCCTCGATGAAGTGGCC
GACTTGCCCTTGGCGATGCAGGTCAAGCTGTTGCGAGCCATCCAGGAAAAAGCCGTGCGCGCCGTTGGCGGCCAACAGGA
AGAAGTGGTGGATGTGCGCATCCTCTGCGCCACCCACAAGGATCTGGACGGCGAAGTGGCTGCCGGGCGCTTTCGCCAGG
ATTTGTATTACCGGCTGAACGTCATCGAACTGCGCGTGCCGCCGTTGCGTGAACGTCGTGAAGACATCGAGCTGCTGGCT
AACCACATGCTCCAGCGCCTGGCGGCCAACACCGGCAGTCCACCGGTCAGATTGCATCCCCAAGCGTTGGACGCTCTCAA
GAATTACCGCTTCCCGGGCAATGTGCGAGAGCTGGAGAACATGCTCGAACGTGCCTACACCCTTTGTGAACAGCAGCAGA
TCGAAGCGGATGACCTGCGCCTGGCCGAGGGCAATAGTACTACTGAGGCAGGCAATCCCGATTTGATGCAGGTCGACAAC
CTGGAGGATTACCTGGAAGACGTTGAGCGCAAGCTCATCCTCCAGGCGTTGGAGGAAACCCGCTGGAACCGCACGGCGGC
GGCGCAGCGGTTGAAGTTGTCGTTTCGGTCGATGCGGTATCGGTTGAAGAAGCTTGGGCTGGATTGA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(405-445)

Sigma-54 interaction domain (Sigma54_activat)

(139-304)

Response regulator receiver domain (Response_reg)

(9-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2KLF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

79.054

99.107

0.783
  pilR Acinetobacter baumannii strain A118

50.327

100

0.516