Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH89_RS25540 Genome accession   NZ_CP117455
Coordinates   5660084..5661118 (-) Length   344 a.a.
NCBI ID   WP_014720327.1    Uniprot ID   A0A0W0MXX4
Organism   Pseudomonas sp. FP1911     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5655084..5666118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH89_RS25510 (PSH89_25505) ruvX 5655202..5655639 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PSH89_RS25515 (PSH89_25510) pyrR 5655772..5656278 (+) 507 WP_019817751.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH89_RS25520 (PSH89_25515) - 5656303..5657307 (+) 1005 WP_014720325.1 aspartate carbamoyltransferase catalytic subunit -
  PSH89_RS25525 (PSH89_25520) - 5657304..5658575 (+) 1272 WP_038451845.1 dihydroorotase -
  PSH89_RS25530 (PSH89_25525) - 5658619..5659047 (-) 429 WP_003195076.1 TM2 domain-containing protein -
  PSH89_RS25535 (PSH89_25530) - 5659297..5659935 (+) 639 WP_003195078.1 C40 family peptidase -
  PSH89_RS25540 (PSH89_25535) pilT 5660084..5661118 (-) 1035 WP_014720327.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH89_RS25545 (PSH89_25540) - 5661176..5661862 (+) 687 WP_032894716.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH89_RS25550 (PSH89_25545) proC 5661897..5662715 (+) 819 WP_038451849.1 pyrroline-5-carboxylate reductase -
  PSH89_RS25555 (PSH89_25550) - 5662741..5663328 (+) 588 WP_003195087.1 YggT family protein -
  PSH89_RS25560 (PSH89_25555) - 5663567..5664718 (+) 1152 WP_034128945.1 homoserine O-acetyltransferase -
  PSH89_RS25565 (PSH89_25560) metW 5664715..5665335 (+) 621 WP_003195091.1 methionine biosynthesis protein MetW -
  PSH89_RS25570 (PSH89_25565) - 5665357..5665791 (+) 435 WP_014720331.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37237.01 Da        Isoelectric Point: 7.2995

>NTDB_id=785573 PSH89_RS25540 WP_014720327.1 5660084..5661118(-) (pilT) [Pseudomonas sp. FP1911]
MDITQLLTASVSRGASDLHLSAGLAPMLRIDGEVWPLDGPALDATQVVDLLSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRAIATEVQSLEALGLGEVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNRHRRQHILTLED
PIEFIHRPKLALVNQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLVSREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785573 PSH89_RS25540 WP_014720327.1 5660084..5661118(-) (pilT) [Pseudomonas sp. FP1911]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCAGACCTGCATCTGTCAGCCGGCCTGGCACCGAT
GCTGCGCATTGATGGCGAGGTTTGGCCGCTGGATGGGCCAGCGCTGGACGCTACCCAAGTGGTCGACTTATTGAGCCCGT
TGCTGAATAAACATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCAGGCGTCGCCCGG
TTCCGGGCGAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCAGTGTTCCGGGCTATTGCGACTGAGGTCCAGAGCCTGGA
GGCGCTGGGATTGGGCGAGGTGTTCCAGCGTATCGCCCGCCTTCCTCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGTT
CTGGCAAGTCCACGACCCTGGCGGCGATGATCGACGACCTCAACCGGCATCGACGCCAGCATATCCTCACGCTGGAAGAC
CCCATCGAGTTTATCCACAGGCCGAAACTGGCGCTGGTCAACCAGCGCCAGGTGCATCGTGACACCCACAGTTTCTCGGT
GGCCCTGCGTTCAGCCCTGCGAGAAGACCCGGATGTGATCCTGGTGGGTGAATTGCGCGATCTGGAAACCATCCGCCTTG
CGCTGACGGCGGCAGAGACCGGGCACCTGGTGTTCGGCACTCTGCACACCTCGTCGGCGGCAAAGACGATAGACCGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCGGTGGTCTCTCAGGT
GTTGGTGAAGAAGGTGGGCGGTGGGCGTGTGGCCGCCCATGAAATCATGCTGGGTACGCCGGCGATTCGTAACTTGATCC
GTGAGGACAAGGTGGCGCAGATGGTTTCGGCAATTCAGACCGGTGGGGCGTTGGGGATGAAGACCCTGGATATGAGTTTG
AAGGCGTTGGTGGGCGCGGGCCTGGTCAGTCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCGGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0MXX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter nosocomialis M2

68.622

99.128

0.68
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.965

0.64
  pilT Vibrio cholerae strain A1552

65.282

97.965

0.64
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Neisseria meningitidis 8013

60.588

98.837

0.599
  pilT Neisseria gonorrhoeae MS11

60.294

98.837

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381