Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PSH72_RS24570 Genome accession   NZ_CP117453
Coordinates   5431424..5433013 (+) Length   529 a.a.
NCBI ID   WP_305503341.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1962     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5426424..5438013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH72_RS24550 (PSH72_24545) pgeF 5428205..5428930 (-) 726 WP_305446947.1 peptidoglycan editing factor PgeF -
  PSH72_RS24555 (PSH72_24550) rluD 5428927..5429889 (-) 963 WP_007901932.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSH72_RS24560 (PSH72_24555) - 5430042..5431064 (+) 1023 WP_305446948.1 outer membrane protein assembly factor BamD -
  PSH72_RS24565 (PSH72_24560) - 5431201..5431434 (+) 234 WP_305446949.1 PP0621 family protein -
  PSH72_RS24570 (PSH72_24565) pilS 5431424..5433013 (+) 1590 WP_305503341.1 PAS domain-containing sensor histidine kinase Regulator
  PSH72_RS24575 (PSH72_24570) pilR 5433016..5434356 (+) 1341 WP_305503343.1 sigma-54 dependent transcriptional regulator Regulator
  PSH72_RS24580 (PSH72_24575) thiO 5434414..5435514 (-) 1101 WP_305503344.1 glycine oxidase ThiO -
  PSH72_RS24585 (PSH72_24580) - 5435571..5435972 (-) 402 WP_305503346.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58626.43 Da        Isoelectric Point: 7.0720

>NTDB_id=785513 PSH72_RS24570 WP_305503341.1 5431424..5433013(+) (pilS) [Pseudomonas cucumis strain FP1962]
MIAETSSPGNKQARRLLRLYHLYRLSVGITLVLLISSNMDNQLLTFANDDLLRNGSWLYLVLNILLVVFLGNTRRPAQLF
SLTLVDVLLLCGLFYAAGGVASAIGNLLIVSVAIGNTLLRGRIGLLIAAVGALGIVGLSFLLSFSHPASPNDYLQVGTLG
ALCFAAALLVQGLIRRLEVSETLAEQRASEVLGLEALNALILQRMRTGILVLDDQRRVQLANHSALTLLGQEKLDGQLID
DHSMPLVERLQLWFNNPTLRPQSLKIASNGLELQPSFIALDQSPHHQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELNGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTVPQRLDLRPWLEHFVAETREGATER
QQIHLHIGSGDFKTLMDPNQLTQILDNLLRNAWRHSALNHDRAQAWLELFIDTDSQLPTLEVLDDGPGVAPDQRSHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=785513 PSH72_RS24570 WP_305503341.1 5431424..5433013(+) (pilS) [Pseudomonas cucumis strain FP1962]
GTGATCGCTGAGACGTCCAGTCCCGGCAACAAACAGGCCCGGCGACTGCTGCGCCTCTATCACCTCTACCGCTTAAGCGT
CGGCATCACCCTGGTGCTGTTGATCTCCAGCAACATGGACAACCAGTTGCTGACGTTCGCCAATGACGACCTGCTGCGCA
ATGGCAGCTGGTTGTACCTGGTGCTGAATATCCTGCTGGTGGTCTTTCTGGGGAACACTCGGCGTCCGGCGCAGCTGTTC
AGCCTGACGCTGGTCGATGTCCTGCTGCTGTGCGGCCTGTTCTACGCCGCAGGAGGCGTCGCCAGCGCCATCGGCAACTT
GCTGATCGTGTCGGTGGCCATCGGCAATACCCTGCTGCGCGGACGAATCGGCCTGTTGATTGCTGCGGTCGGCGCCCTCG
GCATCGTGGGTTTGAGTTTCCTACTGAGCTTCAGCCATCCCGCCAGCCCCAACGATTACCTGCAAGTCGGCACCCTCGGC
GCCCTGTGTTTTGCTGCGGCATTGCTGGTACAAGGCTTGATCCGACGCCTGGAAGTCAGCGAAACCCTCGCCGAGCAGCG
GGCCAGCGAAGTGCTCGGCCTCGAAGCCCTCAACGCACTGATCCTGCAACGCATGCGCACAGGCATTTTGGTGCTCGACG
ACCAACGGCGTGTGCAACTGGCCAATCACAGCGCGTTGACCTTGCTGGGCCAGGAAAAACTCGATGGCCAGTTGATCGAC
GACCACTCGATGCCGTTGGTCGAGCGGCTCCAACTGTGGTTCAACAATCCGACTTTGCGCCCGCAAAGCTTGAAAATCGC
CAGTAATGGGCTAGAGCTGCAACCAAGCTTTATTGCCCTCGACCAAAGCCCGCACCACCAGACGCTGGTTTTTCTCGAAG
ACCTCGCCCAAATCGCCCAACAGGCCCAGCAACTGAAACTTGCCGCCCTCGGTCGCCTGACTGCCGGTATTGCTCATGAG
ATCCGCAATCCACTGGGCGCCATTAGTCATGCCGCGCAGTTACTGCAGGAATCCGAGGAACTGAACGGCGCGGATCGGCG
TCTGACACAGATTATTCAAGACCACTCTCAGCGAATGAATCGGGTAATCGAAAACGTCCTGCAACTGTCCCGTCGCCAGC
AAACCGTGCCACAACGGCTGGATCTGCGACCGTGGCTGGAGCATTTCGTCGCTGAAACCCGCGAAGGGGCGACCGAACGT
CAGCAAATTCACCTGCACATCGGTTCCGGAGACTTCAAAACCCTGATGGATCCGAATCAGCTGACCCAGATTCTCGACAA
TCTGTTACGCAATGCCTGGCGTCATAGCGCCCTGAACCATGATCGGGCGCAGGCCTGGCTTGAGTTGTTTATTGATACGG
ACAGCCAGCTGCCGACCCTTGAAGTCCTGGACGATGGCCCCGGCGTGGCGCCGGACCAGCGCTCGCACTTGTTCGAACCG
TTCTTCACCACCAGCAGCCAGGGTACTGGCCTGGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACTTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.189

100

0.603