Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQP88_RS03835 Genome accession   NZ_CP117390
Coordinates   785213..786193 (+) Length   326 a.a.
NCBI ID   WP_274890522.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Uganda strain RM008     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 780213..791193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQP88_RS03805 (PQP88_03805) - 780790..781797 (+) 1008 WP_000252200.1 DUF1202 domain-containing protein -
  PQP88_RS03810 (PQP88_03810) hemW 781889..783025 (-) 1137 WP_079798516.1 radical SAM family heme chaperone HemW -
  PQP88_RS03815 (PQP88_03815) - 783018..783611 (-) 594 WP_001174764.1 XTP/dITP diphosphatase -
  PQP88_RS03820 (PQP88_03820) yggU 783619..783909 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  PQP88_RS03825 (PQP88_03825) - 783906..784472 (-) 567 WP_001094848.1 YggT family protein -
  PQP88_RS03830 (PQP88_03830) - 784491..785195 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  PQP88_RS03835 (PQP88_03835) pilT 785213..786193 (+) 981 WP_274890522.1 type IV pilus twitching motility protein PilT Machinery gene
  PQP88_RS03840 (PQP88_03840) - 786328..787014 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  PQP88_RS03845 (PQP88_03845) ruvX 787061..787477 (-) 417 WP_001014572.1 Holliday junction resolvase RuvX -
  PQP88_RS03850 (PQP88_03850) - 787477..788040 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  PQP88_RS03855 (PQP88_03855) gshB 788256..789203 (-) 948 WP_000593248.1 glutathione synthase -
  PQP88_RS03860 (PQP88_03860) rsmE 789223..789954 (-) 732 WP_023203985.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PQP88_RS03865 (PQP88_03865) endA 790031..790738 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36163.33 Da        Isoelectric Point: 7.5116

>NTDB_id=784431 PQP88_RS03835 WP_274890522.1 785213..786193(+) (pilT) [Salmonella enterica subsp. enterica serovar Uganda strain RM008]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRPLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=784431 PQP88_RS03835 WP_274890522.1 785213..786193(+) (pilT) [Salmonella enterica subsp. enterica serovar Uganda strain RM008]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCCTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGAGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.082

97.853

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.858

100

0.374
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362