Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   PPW95_RS09995 Genome accession   NZ_CP117039
Coordinates   359016..359594 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain VP220218     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354016..364594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPW95_RS09980 (PPW95_09980) oxyR 354339..355247 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  PPW95_RS09985 (PPW95_09985) - 355303..357873 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  PPW95_RS09990 (PPW95_09990) pilM 358016..359032 (+) 1017 WP_031855783.1 type IV pilus biogenesis protein PilM Machinery gene
  PPW95_RS09995 (PPW95_09995) pilN 359016..359594 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  PPW95_RS10000 (PPW95_10000) pilO 359587..360180 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  PPW95_RS10005 (PPW95_10005) pilP 360170..360685 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  PPW95_RS10010 (PPW95_10010) pilQ 360717..362465 (+) 1749 WP_031855784.1 type IV pilus secretin PilQ Machinery gene
  PPW95_RS10015 (PPW95_10015) aroK 362654..363172 (+) 519 WP_005381319.1 shikimate kinase AroK -
  PPW95_RS10020 (PPW95_10020) aroB 363204..364304 (+) 1101 WP_025794806.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=782171 PPW95_RS09995 WP_015297396.1 359016..359594(+) (pilN) [Vibrio parahaemolyticus strain VP220218]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=782171 PPW95_RS09995 WP_015297396.1 359016..359594(+) (pilN) [Vibrio parahaemolyticus strain VP220218]
GTGCTTTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTATCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594