Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   MWM20_RS18055 Genome accession   NZ_AP025677
Coordinates   3721267..3722004 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain Rb-23     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3716267..3727004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM20_RS18040 (TRECRb23_34330) clpC 3716721..3719294 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  MWM20_RS18045 (TRECRb23_34340) yfiH 3719424..3720155 (-) 732 WP_000040130.1 purine nucleoside phosphorylase YfiH -
  MWM20_RS18050 (TRECRb23_34350) rluD 3720152..3721132 (-) 981 WP_000079112.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MWM20_RS18055 (TRECRb23_34360) comL 3721267..3722004 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  MWM20_RS18060 (TRECRb23_34370) raiA 3722274..3722615 (+) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  MWM20_RS18065 pheL 3722719..3722766 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  MWM20_RS18070 (TRECRb23_34380) pheA 3722865..3724025 (+) 1161 WP_000200140.1 bifunctional chorismate mutase/prephenate dehydratase -
  MWM20_RS18075 (TRECRb23_34390) tyrA 3724068..3725189 (-) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  MWM20_RS18080 (TRECRb23_34400) aroF 3725200..3726270 (-) 1071 WP_001168045.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  MWM20_RS18085 (TRECRb23_34410) yfiL 3726480..3726845 (+) 366 WP_001296308.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=78142 MWM20_RS18055 WP_000197686.1 3721267..3722004(+) (comL) [Escherichia coli strain Rb-23]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=78142 MWM20_RS18055 WP_000197686.1 3721267..3722004(+) (comL) [Escherichia coli strain Rb-23]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCACCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTTGCCGAGTATTATACAGA
ACGTGGCGCATGGGTTGCTGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTATCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment