Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PIB32_RS14145 Genome accession   NZ_CP116898
Coordinates   3386260..3387336 (-) Length   358 a.a.
NCBI ID   WP_308364224.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3381260..3392336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS14120 aroK 3381778..3382302 (-) 525 WP_308368616.1 shikimate kinase AroK -
  PIB32_RS14125 pilQ 3382302..3384521 (-) 2220 WP_308364220.1 type IV pilus secretin PilQ -
  PIB32_RS14130 - 3384524..3385051 (-) 528 WP_308364221.1 pilus assembly protein PilP -
  PIB32_RS14135 - 3385048..3385668 (-) 621 WP_308364222.1 type 4a pilus biogenesis protein PilO -
  PIB32_RS14140 - 3385680..3386258 (-) 579 WP_308364223.1 PilN domain-containing protein -
  PIB32_RS14145 pilM 3386260..3387336 (-) 1077 WP_308364224.1 pilus assembly protein PilM Machinery gene
  PIB32_RS14150 - 3387483..3390134 (+) 2652 WP_308364225.1 penicillin-binding protein 1A -
  PIB32_RS14155 - 3390193..3390675 (-) 483 WP_308364226.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38953.30 Da        Isoelectric Point: 4.3676

>NTDB_id=780968 PIB32_RS14145 WP_308364224.1 3386260..3387336(-) (pilM) [Microbulbifer sp. RZ01]
MGMLPFLNKGPKAMLGLDISSTAVKLLELSRSGDKYRVECYAVEPLPPNAVVDKNINDVAATAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMPADLSEDALEAQIALEADQYIPYPLDEVNLDFEVQGPSEKGEGQVEVLLAACRSENVEQRVSAL
TDAELQAGVVDVEAYAIERAYTLLERQFPPQEQLVVAVIDIGATMSALSVLVDGKTVYTREQLFGGRQLTDEIQRRYGLS
SEEAGLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMDGLVELVGQKLNKPTIV
ANPFQDMTVASRVNRQALANEAPALMIAAGLAMREREY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=780968 PIB32_RS14145 WP_308364224.1 3386260..3387336(-) (pilM) [Microbulbifer sp. RZ01]
ATGGGGATGCTCCCTTTTCTCAACAAGGGCCCGAAGGCCATGCTGGGGCTGGATATCAGCTCCACTGCGGTCAAGTTGCT
GGAGCTCTCCCGCAGCGGCGACAAGTATCGGGTCGAATGTTACGCGGTCGAGCCCCTGCCTCCCAATGCGGTAGTGGACA
AAAACATCAACGATGTTGCCGCCACCGCGGAAGCCATTCGCAAGGTGGTGCGCCGCTCCAAGACGCGCCTGCGTCAGGCT
GCGGTGGCGGTGTCCGGCTCCGCGGTGATCACCAAGACCCTGGAAATGCCCGCCGATCTTTCCGAAGACGCACTTGAGGC
GCAGATCGCCCTGGAGGCGGATCAGTACATTCCCTACCCGCTGGATGAAGTGAATCTCGACTTCGAGGTGCAGGGCCCTT
CGGAAAAAGGCGAAGGCCAGGTGGAGGTGCTGCTGGCCGCATGCCGCAGTGAGAATGTCGAACAGCGGGTTTCCGCGCTG
ACCGACGCCGAACTGCAGGCCGGGGTGGTGGATGTGGAAGCCTACGCCATCGAGCGCGCTTACACCCTGCTGGAACGTCA
GTTCCCCCCCCAGGAACAACTGGTGGTGGCCGTCATCGATATCGGCGCCACCATGAGCGCGCTCTCGGTGCTGGTGGACG
GCAAAACGGTGTACACCCGCGAGCAGCTGTTCGGGGGTCGCCAGCTTACCGACGAGATCCAGCGCCGCTACGGTCTGTCC
TCGGAAGAGGCGGGGCTGGCCAAGCGACAGGGCGGCAGCGGCCTGCCGGACGACTATTACCCGGAGGTGCTGGAGCCCTT
CCGCGATGCCGTGGTGCAGCAGGTCACCCGCTCGCTGCAGTTCTTCTACTCCTCCACTTCCTACAGCGATGTGGATTACA
TATTGCTCGGCGGCGGCGTTGCGGCCATGGACGGGCTGGTGGAACTGGTGGGGCAGAAGCTCAACAAACCCACGATTGTC
GCCAATCCCTTTCAGGATATGACAGTGGCCTCACGAGTCAATCGCCAGGCGCTGGCCAACGAGGCGCCGGCGCTGATGAT
CGCCGCCGGCCTGGCGATGCGCGAGCGGGAGTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

98.324

0.503
  comM Acinetobacter nosocomialis M2

51.136

98.324

0.503
  comM Acinetobacter baylyi ADP1

47.159

98.324

0.464
  pilM Legionella pneumophila strain ERS1305867

43.539

99.441

0.433