Detailed information NTG0780217

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PKO50_RS12385
• Genome accession: NZ_CP116775
• Coordinates: 2605604..2606806 (+)
• Length: 400 a.a.
• NCBI ID: WP_177412080.1
• Uniprot ID: -
• Organism: Pseudomonas sp. 273
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2600604..2611806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKO50_RS12360	-	2601304..2602584 (-)	1281	WP_109934951.1	OprD family porin	-
PKO50_RS12365	-	2602825..2604024 (-)	1200	WP_275630469.1	MFS transporter	-
PKO50_RS12370	-	2604202..2604759 (-)	558	WP_275629868.1	putative glycolipid-binding domain-containing protein	-
PKO50_RS12375	-	2604756..2605118 (-)	363	WP_275629869.1	MmcQ/YjbR family DNA-binding protein	-
PKO50_RS12380	-	2605132..2605410 (-)	279	WP_109934947.1	peptidylprolyl isomerase	-
PKO50_RS12385	pilU	2605604..2606806 (+)	1203	WP_177412080.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PKO50_RS12390	-	2607176..2608432 (-)	1257	WP_275629870.1	OprD family porin	-
PKO50_RS12395	-	2608829..2609605 (+)	777	WP_275629871.1	IclR family transcriptional regulator	-
PKO50_RS12400	-	2609802..2610854 (+)	1053	WP_275629872.1	Gfo/Idh/MocA family oxidoreductase	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 43878.01 Da
• Isoelectric Point: 6.0779

>NTDB_id=780217 PKO50_RS12385 WP_177412080.1 2605604..2606806(+) (pilU) [Pseudomonas sp. 273]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLMLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPASATAQAAGTQPAAPAAPAVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide

• Length: 1203 bp

>NTDB_id=780217 PKO50_RS12385 WP_177412080.1 2605604..2606806(+) (pilU) [Pseudomonas sp. 273]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAGCCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAGCAGAAGGCCGAGTTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGACGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATAGTCGCGCGCAACATCAAGCTGGACATCCCGCGCTTCGA
GGACCTGATGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGATACCGGGCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAACGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACCCAGGATGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAGATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAATCCGGCCAGCGCCACGGCCCAGGCGGCCGGCACCCAGCCGGCGGCCCCGGCCGCACCCG
CGGTGGAAGCCTCCAGCTGGGGCCTGGAGCTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	87.75	0.495
pilU	Acinetobacter baylyi ADP1	53.409	88	0.47
pilU	Vibrio cholerae strain A1552	51.24	90.75	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.42	95.25	0.385