Detailed information NTG0780197

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: PNF30_RS14010
• Genome accession: NZ_CP116774
• Coordinates: 2716751..2720455 (-)
• Length: 1234 a.a.
• NCBI ID: WP_272510308.1
• Uniprot ID: -
• Organism: Bacillus safensis strain SRCM125915
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2711751..2725455

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PNF30_RS13995 (PNF30_13995)	-	2711838..2712137 (-)	300	WP_048238424.1	hypothetical protein	-
PNF30_RS14000 (PNF30_14000)	-	2712157..2715552 (-)	3396	WP_272510307.1	AAA family ATPase	-
PNF30_RS14005 (PNF30_14005)	sbcD	2715549..2716724 (-)	1176	WP_144470097.1	exonuclease subunit SbcD	-
PNF30_RS14010 (PNF30_14010)	addA	2716751..2720455 (-)	3705	WP_272510308.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
PNF30_RS14015 (PNF30_14015)	addB	2720433..2723942 (-)	3510	WP_197198448.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
PNF30_RS14020 (PNF30_14020)	-	2724096..2724542 (+)	447	WP_048238419.1	ferritin-like domain-containing protein	-
PNF30_RS14025 (PNF30_14025)	-	2724740..2725150 (-)	411	WP_025093362.1	DoxX family protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142235.58 Da
• Isoelectric Point: 5.1733

>NTDB_id=780197 PNF30_RS14010 WP_272510308.1 2716751..2720455(-) (addA) [Bacillus safensis strain SRCM125915]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPENPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDVFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFKPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLEQFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEETGNVVLSHPSVFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
TTLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLPSDVLYDDSRIQQLLDSLQQRDLLTNEQVQSIDQEGITAFFSTSIGQ
KLQKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLKAAAPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=780197 PNF30_RS14010 WP_272510308.1 2716751..2720455(-) (addA) [Bacillus safensis strain SRCM125915]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAAATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAACATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCACTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGCTTGCAGGTTCTGCGCACCTTTTATTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACGTCTGACCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCCTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTATCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTTGAACGAGCT
CTCGCTCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCGGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
GATCAGGCATCAAGATGTTTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAACTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTTATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTAAACCAGATGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACAAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTCCTTGTTCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGGGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTAGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAATTTGCAGTATCGGGACAT
TGTGATTTTGCTCCGCTCTATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGGATTCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCTATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAGAAAGGCACTTATTATGATGCTGTGAAAGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACAATTTTTCAGACTGCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGCGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTG
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGACTGGCTGAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGACCTGCTCTTATCCGTCACCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGTAATGTCGTTCTGTCGCATCCATCAGTATTCACGATCTCATTTACTCAAGCTTCTGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATATCCATATTTAGCGGCCTCACAAGTCGGTACAA
AGCAGTCTGTTTCTGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
ACAACGTTGTATGACCGTCCGGCTTTCATGAAGAAAAAAGCACTGACTGCTGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTCCGCTTCCTTCTGATGTGCTCTATGATGATTCACGTATACAGCAACTGCTCGATTCCTTACAGCAGA
GAGACTTACTAACAAATGAACAAGTCCAGTCAATTGATCAAGAAGGAATTACAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCAAAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGAGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATATACAGGCGGCCTTAAAGCAGCTGCGCCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
CGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.181	100	0.663