Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   PNF30_RS14015 Genome accession   NZ_CP116774
Coordinates   2720433..2723942 (-) Length   1169 a.a.
NCBI ID   WP_197198448.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2715433..2728942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS14005 (PNF30_14005) sbcD 2715549..2716724 (-) 1176 WP_144470097.1 exonuclease subunit SbcD -
  PNF30_RS14010 (PNF30_14010) addA 2716751..2720455 (-) 3705 WP_272510308.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  PNF30_RS14015 (PNF30_14015) addB 2720433..2723942 (-) 3510 WP_197198448.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  PNF30_RS14020 (PNF30_14020) - 2724096..2724542 (+) 447 WP_048238419.1 ferritin-like domain-containing protein -
  PNF30_RS14025 (PNF30_14025) - 2724740..2725150 (-) 411 WP_025093362.1 DoxX family protein -
  PNF30_RS14030 (PNF30_14030) - 2725208..2726047 (-) 840 WP_048238418.1 M56 family metallopeptidase -
  PNF30_RS14035 (PNF30_14035) - 2726040..2726462 (-) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  PNF30_RS14040 (PNF30_14040) - 2726729..2727874 (+) 1146 WP_111290180.1 AbrB family transcriptional regulator -
  PNF30_RS14045 (PNF30_14045) lepB 2727912..2728424 (-) 513 WP_047202354.1 signal peptidase I -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134807.83 Da        Isoelectric Point: 5.3937

>NTDB_id=780198 PNF30_RS14015 WP_197198448.1 2720433..2723942(-) (addB) [Bacillus safensis strain SRCM125915]
MEIQFLAGRSGSGKTTAILEEIKEQLRHDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLARECGAAISENILEGNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGAAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIKVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLETGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=780198 PNF30_RS14015 WP_197198448.1 2720433..2723942(-) (addB) [Bacillus safensis strain SRCM125915]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCATGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGCGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACGGAAGAAAGACGGGCAGCTGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCCGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACTGCTGCGTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCAAG
AGAGTGCGGAGCGGCCATTTCTGAGAACATTTTGGAGGGAAATAACCGTCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAACCATATCAGGAAGTCACCCCTCATCTCACAGTATCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAGGGAGCAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGGCTCAGACTGCGGGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTATGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGACGAT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCCCCGCTTTTCCAGTTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTCAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATAAAAGTGCCGGAAAAACTTGAAAAGGCCAGGCTGGAGGCTGAGGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTCATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGACTCGCCTCTCTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGCATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACCGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTCGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGCTTTCGAGTCCATCCCATCATTTGTATCT
GTCTTATTCGGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCGA
ATCATCAGCATGCTTTGTATGTGTTAGACCCAGAACAGCTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCTTATACCGTCTCACAGCTGCAACAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTTTCGAAAAATATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCGAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCGTTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAAGACCGGCAATTTTATAAACTGGAAGCACCTGATATCGG
CCAGCTGTTTCACTCAGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGACTGTGTCACTTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATCTTAAGTGAGCATGCAAAAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGTCAAGGACCTTTGCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATATTATGGACTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAGGAATGGCTCGGCGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAAGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAAACAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTCGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658