Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PNF30_RS07695 Genome accession   NZ_CP116774
Coordinates   1507408..1508478 (+) Length   356 a.a.
NCBI ID   WP_272510912.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1502408..1513478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS07660 (PNF30_07660) - 1503542..1503856 (+) 315 WP_048238883.1 MTH1187 family thiamine-binding protein -
  PNF30_RS07665 (PNF30_07665) - 1504080..1504253 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  PNF30_RS07670 (PNF30_07670) - 1504411..1505049 (+) 639 WP_272510911.1 MBL fold metallo-hydrolase -
  PNF30_RS07675 (PNF30_07675) - 1505296..1505538 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  PNF30_RS07680 (PNF30_07680) - 1505751..1506131 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PNF30_RS07690 (PNF30_07690) - 1506355..1507230 (+) 876 WP_095409007.1 STAS domain-containing protein -
  PNF30_RS07695 (PNF30_07695) comGA 1507408..1508478 (+) 1071 WP_272510912.1 competence type IV pilus ATPase ComGA Machinery gene
  PNF30_RS07700 (PNF30_07700) comGB 1508459..1509499 (+) 1041 WP_272510913.1 competence type IV pilus assembly protein ComGB -
  PNF30_RS07705 (PNF30_07705) comGC 1509516..1509809 (+) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  PNF30_RS07710 (PNF30_07710) comGD 1509802..1510248 (+) 447 WP_272510914.1 competence type IV pilus minor pilin ComGD -
  PNF30_RS07715 (PNF30_07715) comGE 1510232..1510546 (+) 315 WP_111292285.1 competence type IV pilus minor pilin ComGE -
  PNF30_RS07720 (PNF30_07720) comGF 1510458..1510985 (+) 528 WP_186318660.1 competence type IV pilus minor pilin ComGF -
  PNF30_RS07725 (PNF30_07725) - 1510982..1511365 (+) 384 WP_272510915.1 competence type IV pilus minor pilin ComGG -
  PNF30_RS07730 (PNF30_07730) - 1511425..1511619 (+) 195 WP_197198253.1 YqzE family protein -
  PNF30_RS07735 (PNF30_07735) - 1511653..1511931 (-) 279 WP_223251017.1 DUF3889 domain-containing protein -
  PNF30_RS07740 (PNF30_07740) tapA 1512230..1512757 (+) 528 WP_272510916.1 amyloid fiber anchoring/assembly protein TapA -
  PNF30_RS07745 (PNF30_07745) sipW 1512810..1513382 (+) 573 WP_272510917.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40454.02 Da        Isoelectric Point: 9.6868

>NTDB_id=780175 PNF30_RS07695 WP_272510912.1 1507408..1508478(+) (comGA) [Bacillus safensis strain SRCM125915]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPYRRTNVFELLYGKELGKCIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=780175 PNF30_RS07695 WP_272510912.1 1507408..1508478(+) (comGA) [Bacillus safensis strain SRCM125915]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCAGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCTAGATTATTATCATTTTTGA
AGCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATACTCATTAATCCAGTTT
GCAAAAAAGAATTTTAATCGAAATATTATTACCCTTGAAGACCCTGTCGAAACGAGGAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTGG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTACAGACGAACAAATGTATTTGAATTGCTTTATGGAAAGGAGCTTGGTAAGTGTATCAAAGAGGCCAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649