Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   PNF30_RS07705 Genome accession   NZ_CP116774
Coordinates   1509516..1509809 (+) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain SRCM125915     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504516..1514809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS07675 (PNF30_07675) - 1505296..1505538 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  PNF30_RS07680 (PNF30_07680) - 1505751..1506131 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PNF30_RS07690 (PNF30_07690) - 1506355..1507230 (+) 876 WP_095409007.1 STAS domain-containing protein -
  PNF30_RS07695 (PNF30_07695) comGA 1507408..1508478 (+) 1071 WP_272510912.1 competence type IV pilus ATPase ComGA Machinery gene
  PNF30_RS07700 (PNF30_07700) comGB 1508459..1509499 (+) 1041 WP_272510913.1 competence type IV pilus assembly protein ComGB -
  PNF30_RS07705 (PNF30_07705) comGC 1509516..1509809 (+) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  PNF30_RS07710 (PNF30_07710) comGD 1509802..1510248 (+) 447 WP_272510914.1 competence type IV pilus minor pilin ComGD -
  PNF30_RS07715 (PNF30_07715) comGE 1510232..1510546 (+) 315 WP_111292285.1 competence type IV pilus minor pilin ComGE -
  PNF30_RS07720 (PNF30_07720) comGF 1510458..1510985 (+) 528 WP_186318660.1 competence type IV pilus minor pilin ComGF -
  PNF30_RS07725 (PNF30_07725) - 1510982..1511365 (+) 384 WP_272510915.1 competence type IV pilus minor pilin ComGG -
  PNF30_RS07730 (PNF30_07730) - 1511425..1511619 (+) 195 WP_197198253.1 YqzE family protein -
  PNF30_RS07735 (PNF30_07735) - 1511653..1511931 (-) 279 WP_223251017.1 DUF3889 domain-containing protein -
  PNF30_RS07740 (PNF30_07740) tapA 1512230..1512757 (+) 528 WP_272510916.1 amyloid fiber anchoring/assembly protein TapA -
  PNF30_RS07745 (PNF30_07745) sipW 1512810..1513382 (+) 573 WP_272510917.1 signal peptidase I SipW -
  PNF30_RS07750 (PNF30_07750) tasA 1513437..1514237 (+) 801 WP_111292280.1 biofilm matrix protein TasA -
  PNF30_RS07755 (PNF30_07755) sinR 1514305..1514640 (-) 336 WP_024422969.1 transcriptional regulator SinR Regulator

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=780176 PNF30_RS07705 WP_024422977.1 1509516..1509809(+) (comGC) [Bacillus safensis strain SRCM125915]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=780176 PNF30_RS07705 WP_024422977.1 1509516..1509809(+) (comGC) [Bacillus safensis strain SRCM125915]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTCAAATCAATGGTCAGTACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.222

92.784

0.392
  comGC Staphylococcus aureus N315

42.222

92.784

0.392