Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PNF29_RS19470 Genome accession   NZ_CP116773
Coordinates   3689819..3690268 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain SRCM125727     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3684819..3695268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS19450 (PNF29_19450) hxlR 3686030..3686392 (-) 363 WP_017696131.1 transcriptional activator HxlR -
  PNF29_RS19455 (PNF29_19455) hxlA 3686624..3687256 (+) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  PNF29_RS19460 (PNF29_19460) hxlB 3687262..3687819 (+) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  PNF29_RS19465 (PNF29_19465) tlpC 3687930..3689651 (+) 1722 Protein_3825 methyl-accepting chemotaxis protein TlpC -
  PNF29_RS19470 (PNF29_19470) nucA/comI 3689819..3690268 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  PNF29_RS19475 (PNF29_19475) nin/comJ 3690295..3690693 (+) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  PNF29_RS19480 (PNF29_19480) bglC 3690730..3692163 (-) 1434 WP_017696125.1 6-phospho-beta-glucosidase -
  PNF29_RS19485 (PNF29_19485) yckD 3692317..3692649 (-) 333 WP_015252849.1 YckD family protein -
  PNF29_RS19490 (PNF29_19490) yckC 3692731..3693186 (-) 456 WP_144454062.1 RDD family protein -
  PNF29_RS19495 (PNF29_19495) - 3693205..3693395 (-) 191 Protein_3831 hypothetical protein -
  PNF29_RS19500 (PNF29_19500) yckB 3693558..3694421 (+) 864 WP_072557073.1 transporter substrate-binding domain-containing protein -
  PNF29_RS19505 (PNF29_19505) yckA 3694431..3695111 (+) 681 WP_017696121.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=780143 PNF29_RS19470 WP_014475770.1 3689819..3690268(+) (nucA/comI) [Bacillus subtilis strain SRCM125727]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=780143 PNF29_RS19470 WP_014475770.1 3689819..3690268(+) (nucA/comI) [Bacillus subtilis strain SRCM125727]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987