Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PND17_RS08345 Genome accession   NZ_CP116772
Coordinates   1580977..1582296 (+) Length   439 a.a.
NCBI ID   WP_011225516.1    Uniprot ID   Q5M5T4
Organism   Streptococcus thermophilus strain strain TSGB 4141     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1575977..1587296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PND17_RS08320 (PND17_08320) - 1576831..1577595 (+) 765 WP_002947187.1 ABC transporter ATP-binding protein -
  PND17_RS08325 (PND17_08325) - 1577595..1578305 (+) 711 WP_271298456.1 ABC transporter ATP-binding protein -
  PND17_RS08330 (PND17_08330) - 1578441..1579101 (+) 661 Protein_1624 CBS and ACT domain-containing protein -
  PND17_RS08335 (PND17_08335) cysK 1579268..1580194 (-) 927 WP_271298457.1 cysteine synthase A -
  PND17_RS08340 (PND17_08340) - 1580296..1580922 (-) 627 WP_271298458.1 YigZ family protein -
  PND17_RS08345 (PND17_08345) comFA/cflA 1580977..1582296 (+) 1320 WP_011225516.1 DEAD/DEAH box helicase Machinery gene
  PND17_RS08350 (PND17_08350) - 1582277..1582939 (+) 663 WP_002949747.1 ComF family protein -
  PND17_RS08355 (PND17_08355) hpf 1583018..1583566 (+) 549 WP_022096635.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PND17_RS08360 (PND17_08360) - 1583631..1584272 (-) 642 Protein_1630 transposase -
  PND17_RS08365 (PND17_08365) gshAB 1584374..1586638 (-) 2265 WP_004198358.1 bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50628.72 Da        Isoelectric Point: 9.5663

>NTDB_id=780067 PND17_RS08345 WP_011225516.1 1580977..1582296(+) (comFA/cflA) [Streptococcus thermophilus strain strain TSGB 4141]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHYLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCREEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=780067 PND17_RS08345 WP_011225516.1 1580977..1582296(+) (comFA/cflA) [Streptococcus thermophilus strain strain TSGB 4141]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATTACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGGAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M5T4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae D39

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae R6

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae TIGR4

54.988

98.178

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

54.651

97.95

0.535
  comFA/cflA Streptococcus mitis SK321

53.721

97.95

0.526
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367