Detailed information NTG0779757

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PMJ84_RS02310
• Genome accession: NZ_CP116720
• Coordinates: 494520..495668 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2866
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 489520..500668

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMJ84_RS02285 (PMJ84_02285)	-	490671..490967 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PMJ84_RS02290 (PMJ84_02290)	-	490964..491557 (-)	594	WP_003084544.1	YggT family protein	-
PMJ84_RS02295 (PMJ84_02295)	proC	491568..492389 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PMJ84_RS02300 (PMJ84_02300)	-	492401..493093 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
PMJ84_RS02305 (PMJ84_02305)	pilT	493308..494342 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PMJ84_RS02310 (PMJ84_02310)	pilU	494520..495668 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PMJ84_RS02315 (PMJ84_02315)	czcD	495676..496575 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PMJ84_RS02320 (PMJ84_02320)	-	496729..497133 (+)	405	WP_003084560.1	NINE protein	-
PMJ84_RS02325 (PMJ84_02325)	-	497376..498749 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PMJ84_RS02330 (PMJ84_02330)	-	498746..499930 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=779757 PMJ84_RS02310 WP_003100959.1 494520..495668(+) (pilU) [Pseudomonas aeruginosa strain 2866]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=779757 PMJ84_RS02310 WP_003100959.1 494520..495668(+) (pilU) [Pseudomonas aeruginosa strain 2866]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382