Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PJW01_RS03195 Genome accession   NZ_CP116325
Coordinates   572618..574444 (+) Length   608 a.a.
NCBI ID   WP_000003348.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain BLB1     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 567618..579444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW01_RS03175 (PJW01_03175) - 567921..568595 (-) 675 WP_000362615.1 TerC family protein -
  PJW01_RS03180 (PJW01_03180) mecA 568941..569624 (+) 684 WP_000350716.1 adaptor protein MecA Regulator
  PJW01_RS03185 (PJW01_03185) - 569697..571241 (+) 1545 WP_000799197.1 cardiolipin synthase -
  PJW01_RS03190 (PJW01_03190) - 571322..572566 (+) 1245 WP_000628331.1 competence protein CoiA -
  PJW01_RS03195 (PJW01_03195) pepF 572618..574444 (+) 1827 WP_000003348.1 oligoendopeptidase F Regulator
  PJW01_RS03200 (PJW01_03200) - 574575..574748 (-) 174 WP_000750355.1 hypothetical protein -
  PJW01_RS03205 (PJW01_03205) - 574978..575871 (-) 894 WP_000360656.1 ClpXP adapter SpxH family protein -
  PJW01_RS03210 (PJW01_03210) - 575871..576269 (-) 399 WP_000043386.1 hypothetical protein -
  PJW01_RS03215 (PJW01_03215) - 576438..577016 (-) 579 WP_000191125.1 CYTH domain-containing protein -
  PJW01_RS03220 (PJW01_03220) - 577161..577532 (+) 372 WP_001180006.1 hypothetical protein -
  PJW01_RS03225 (PJW01_03225) - 577563..578201 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  PJW01_RS03230 (PJW01_03230) - 578220..579017 (+) 798 WP_000673190.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70199.57 Da        Isoelectric Point: 4.6443

>NTDB_id=776604 PJW01_RS03195 WP_000003348.1 572618..574444(+) (pepF) [Bacillus thuringiensis strain BLB1]
MSEQNKAKALPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDPRVRKDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=776604 PJW01_RS03195 WP_000003348.1 572618..574444(+) (pepF) [Bacillus thuringiensis strain BLB1]
ATGTCTGAACAAAACAAAGCGAAAGCATTACCAGATCGCAACGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACGGATGCAGAATGGGAAAAAGAATTTCAAGCTATTAAAGAGCTATTGCCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTTCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAAGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGCAATACATTCGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGGGAAGACGGAGAAGAAATAGAAATTACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCCTCGTGTTCGCAAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATTTAAAAACACATTCGCAAGTACG
TTAAGTGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCATTGAGCAA
TAATAATATTCCGGAAGCAGTATACGATCAACTCGTTGAAACAGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGATATTTTGAA
AGAGGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCTAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGGTTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGACATGCTAACGGAAATTTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGATGAAGAGATTGGTTTAGAATGGTCTCGTATTCCACACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTATCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCACTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488