Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   PF996_RS08435 Genome accession   NZ_CP116014
Coordinates   1664485..1665384 (+) Length   299 a.a.
NCBI ID   WP_017417773.1    Uniprot ID   A0A411A614
Organism   Bacillus velezensis strain SRCM124633     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1659485..1670384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF996_RS08415 (PF996_08415) - 1660176..1661879 (+) 1704 WP_017417772.1 hypothetical protein -
  PF996_RS08420 (PF996_08420) - 1661876..1662157 (+) 282 WP_007409769.1 FlhB-like flagellar biosynthesis protein -
  PF996_RS08425 (PF996_08425) sucC 1662332..1663489 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  PF996_RS08430 (PF996_08430) sucD 1663518..1664420 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  PF996_RS08435 (PF996_08435) dprA 1664485..1665384 (+) 900 WP_017417773.1 DNA-processing protein DprA Machinery gene
  PF996_RS08440 (PF996_08440) topA 1665566..1667641 (+) 2076 WP_065180268.1 type I DNA topoisomerase -
  PF996_RS08445 (PF996_08445) trmFO 1667706..1669013 (+) 1308 WP_271265764.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  PF996_RS08450 (PF996_08450) xerC 1669083..1670000 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32834.00 Da        Isoelectric Point: 8.4593

>NTDB_id=775258 PF996_RS08435 WP_017417773.1 1664485..1665384(+) (dprA) [Bacillus velezensis strain SRCM124633]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTALSEGKTVPEAIFREIERKDPELDEVLSDYRRKGITVI
PISSTRYPTWLKAIYDPPAVLYAKGNTPLLEKGRKLGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=775258 PF996_RS08435 WP_017417773.1 1664485..1665384(+) (dprA) [Bacillus velezensis strain SRCM124633]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGCTTTATCAGAAGGGAAAACAGTGCCCGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCAAAGGCATTACTGTCATT
CCGATTTCATCAACCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCCGCTTCTTGAAAAAGGGAGAAAACTCGGGATTGTAGGAACGCGGAAACCGACAGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCATTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A614

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.234

100

0.702
  dprA Legionella pneumophila strain ERS1305867

41.948

89.298

0.375
  dprA Lactococcus lactis subsp. cremoris KW2

41.667

88.294

0.368