Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PF977_RS20745 Genome accession   NZ_CP116012
Coordinates   3935402..3935851 (+) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain SRCM124333     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3930402..3940851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS20725 (PF977_20725) hxlR 3931612..3931974 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  PF977_RS20730 (PF977_20730) hxlA 3932207..3932839 (+) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  PF977_RS20735 (PF977_20735) hxlB 3932845..3933402 (+) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  PF977_RS20740 (PF977_20740) tlpC 3933513..3935234 (+) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  PF977_RS20745 (PF977_20745) nucA/comI 3935402..3935851 (+) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  PF977_RS20750 (PF977_20750) nin/comJ 3935878..3936276 (+) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  PF977_RS20755 (PF977_20755) bglC 3936313..3937746 (-) 1434 WP_003234614.1 6-phospho-beta-glucosidase -
  PF977_RS20760 (PF977_20760) yckD 3937900..3938232 (-) 333 WP_015252849.1 YckD family protein -
  PF977_RS20765 (PF977_20765) - 3938314..3938661 (-) 348 WP_003234619.1 RDD family protein -
  PF977_RS20770 (PF977_20770) - 3938668..3938863 (-) 196 Protein_4084 hypothetical protein -
  PF977_RS20775 (PF977_20775) yckB 3939025..3939888 (+) 864 WP_015715225.1 transporter substrate-binding domain-containing protein -
  PF977_RS20780 (PF977_20780) yckA 3939898..3940578 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=775143 PF977_RS20745 WP_003234608.1 3935402..3935851(+) (nucA/comI) [Bacillus subtilis strain SRCM124333]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=775143 PF977_RS20745 WP_003234608.1 3935402..3935851(+) (nucA/comI) [Bacillus subtilis strain SRCM124333]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98