Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QU520_RS09650 Genome accession   NZ_CP128613
Coordinates   1995647..1996657 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain WL297     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1990647..2001657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU520_RS09615 (QU520_09615) nudB 1990938..1991390 (+) 453 WP_077626310.1 dihydroneopterin triphosphate diphosphatase -
  QU520_RS09620 (QU520_09620) yebC 1991419..1992159 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QU520_RS09625 (QU520_09625) ruvC 1992194..1992715 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QU520_RS09630 (QU520_09630) - 1992717..1993280 (-) 564 WP_234761061.1 YebB family permuted papain-like enzyme -
  QU520_RS09635 (QU520_09635) - 1993188..1994237 (+) 1050 WP_234761074.1 hypothetical protein -
  QU520_RS09640 (QU520_09640) - 1994275..1994859 (+) 585 WP_001054410.1 hypothetical protein -
  QU520_RS09645 (QU520_09645) ruvA 1995027..1995638 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QU520_RS09650 (QU520_09650) ruvB 1995647..1996657 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QU520_RS09655 (QU520_09655) znuB 1996804..1997589 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  QU520_RS09660 (QU520_09660) znuC 1997586..1998341 (-) 756 WP_000202976.1 zinc ABC transporter ATP-binding protein ZnuC -
  QU520_RS09665 (QU520_09665) znuA 1998420..1999352 (+) 933 WP_024212292.1 zinc ABC transporter substrate-binding protein ZnuA -
  QU520_RS09670 (QU520_09670) mepM 1999368..2000690 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=775104 QU520_RS09650 WP_000568522.1 1995647..1996657(+) (ruvB) [Escherichia coli strain WL297]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=775104 QU520_RS09650 WP_000568522.1 1995647..1996657(+) (ruvB) [Escherichia coli strain WL297]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGTTTAGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTGAATTTACGCACGACTTCCGGTCCAGTACTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATTATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGACTTGCCGCCGTTTACTCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGCTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGTAGTGCCCGTTTTATGGGGCTTGAGATGAGTGACGATGGCGCGTTGGAAGTTGCTC
GCCGGGCGCGTGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGTGTTCGCGATTTTGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCGGATATCGCCGCCCAGGCACTGGATATGCTGAATGTTGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGCCTGGATAACCTGGCGGCAGCCATTGGTGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCTTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494