Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   PF976_RS12795 Genome accession   NZ_CP116011
Coordinates   2461583..2462350 (-) Length   255 a.a.
NCBI ID   WP_014470678.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2456583..2467350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS12775 (PF976_12775) - 2456785..2457597 (-) 813 WP_013352914.1 pyruvate, water dikinase regulatory protein -
  PF976_RS12780 (PF976_12780) ccpN 2457623..2458261 (-) 639 WP_014470676.1 transcriptional regulator CcpN -
  PF976_RS12785 (PF976_12785) glyS 2458362..2460401 (-) 2040 WP_014470677.1 glycine--tRNA ligase subunit beta -
  PF976_RS12790 (PF976_12790) glyQ 2460394..2461281 (-) 888 WP_013352917.1 glycine--tRNA ligase subunit alpha -
  PF976_RS12795 (PF976_12795) recO 2461583..2462350 (-) 768 WP_014470678.1 DNA repair protein RecO Machinery gene
  PF976_RS12800 (PF976_12800) - 2462387..2462530 (-) 144 WP_007408286.1 YqzL family protein -
  PF976_RS12805 (PF976_12805) era 2462685..2463590 (-) 906 WP_014470680.1 GTPase Era -
  PF976_RS12810 (PF976_12810) - 2463571..2463981 (-) 411 WP_013352920.1 cytidine deaminase -
  PF976_RS12815 (PF976_12815) - 2464104..2464478 (-) 375 WP_014470681.1 diacylglycerol kinase family protein -
  PF976_RS12820 (PF976_12820) ybeY 2464459..2464932 (-) 474 WP_013352922.1 rRNA maturation RNase YbeY -
  PF976_RS12825 (PF976_12825) - 2464933..2467065 (-) 2133 WP_013352923.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29300.98 Da        Isoelectric Point: 9.0137

>NTDB_id=775043 PF976_RS12795 WP_014470678.1 2461583..2462350(-) (recO) [Bacillus amyloliquefaciens strain SRCM124317]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSRDG
TFHFSVRDNGFICHRCFGKDPYKVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNELKRVIDLYYEEYSGLYLKSKRFL
DQMESMKHLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=775043 PF976_RS12795 WP_014470678.1 2461583..2462350(-) (recO) [Bacillus amyloliquefaciens strain SRCM124317]
ATGCTGACAAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGAGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTTATGGCAAGAGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGACTTGTTTTTGACGGCATATGCCGCTTTTATCGCTGAACTTACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGAAGGGACTGACCCGGACGTGATCACCTTCA
TCGTTCAGATGAAAATGCTCGGCGTCATGGGACTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCCGGGACGGG
ACGTTTCATTTTTCGGTAAGAGATAATGGTTTTATCTGCCACCGCTGCTTTGGAAAGGATCCGTACAAAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCATTAAAACAGGAAA
CCAAAAACGAACTTAAACGGGTTATCGATCTGTATTATGAGGAATATTCAGGGCTTTATTTAAAGTCTAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGCACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

90.196

100

0.902