Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OR232_RS19985 Genome accession   NZ_CP128596
Coordinates   4121458..4121898 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain muskdeer13-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4116458..4126898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OR232_RS19965 (OR232_019930) aroP 4117458..4118828 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  OR232_RS19970 (OR232_019935) ampE 4118871..4119725 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  OR232_RS19975 (OR232_019940) ampD 4119722..4120273 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OR232_RS19980 (OR232_019945) nadC 4120361..4121254 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OR232_RS19985 (OR232_019950) pilA 4121458..4121898 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  OR232_RS19990 (OR232_019955) pilB 4121908..4123293 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  OR232_RS19995 (OR232_019960) hofC 4123283..4124485 (+) 1203 WP_000157292.1 protein transport protein HofC -
  OR232_RS20000 (OR232_019965) guaC 4124520..4125563 (-) 1044 WP_001217343.1 GMP reductase -
  OR232_RS20005 (OR232_019970) - 4125719..4125763 (-) 45 WP_120795372.1 protein YacM -
  OR232_RS20010 (OR232_019975) coaE 4125788..4126408 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=774962 OR232_RS19985 WP_000360913.1 4121458..4121898(+) (pilA) [Escherichia coli strain muskdeer13-4]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=774962 OR232_RS19985 WP_000360913.1 4121458..4121898(+) (pilA) [Escherichia coli strain muskdeer13-4]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363