NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	PG904_RS13685	Genome accession	NZ_CP115941
Coordinates	2978188..2979069 (+)	Length	293 a.a.
NCBI ID	WP_078222781.1	Uniprot ID	-
Organism	Ralstonia syzygii strain BDBR229
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2973188..2984069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PG904_RS13670	-	2973622..2974899 (+)	1278	WP_013211407.1	HlyC/CorC family transporter	-
PG904_RS13675	pilF	2975147..2976874 (+)	1728	WP_078222783.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PG904_RS13680	-	2976920..2978185 (+)	1266	WP_078222782.1	type II secretion system F family protein	-
PG904_RS13685	pilD	2978188..2979069 (+)	882	WP_078222781.1	prepilin peptidase	Machinery gene
PG904_RS13690	coaE	2979082..2979696 (+)	615	WP_078222780.1	dephospho-CoA kinase	-
PG904_RS13695	zapD	2979814..2980572 (+)	759	WP_013211402.1	cell division protein ZapD	-
PG904_RS13700	-	2980584..2980799 (+)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
PG904_RS13705	-	2980796..2981254 (-)	459	WP_048817320.1	NUDIX domain-containing protein	-
PG904_RS13710	-	2981232..2982116 (-)	885	WP_013211399.1	ATP-binding protein	-
PG904_RS13715	argJ	2982132..2983361 (-)	1230	WP_078222779.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

Download Length: 293 a.a. Molecular weight: 31663.68 Da Isoelectric Point: 8.0336

                                >NTDB_id=774533 PG904_RS13685 WP_078222781.1 2978188..2979069(+) (pilD) [Ralstonia syzygii strain BDBR229]

MSGMFVIPTLAQLPAWFVIGSAALLGLLIGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH

QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD

AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS

VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

Download Length: 882 bp

                                >NTDB_id=774533 PG904_RS13685 WP_078222781.1 2978188..2979069(+) (pilD) [Ralstonia syzygii strain BDBR229]

ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT

GCTGATCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG

AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC

CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT

TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG

TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCTGAT

GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT

CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT

ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG

GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT

CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT

AA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(24-129)

Type IV leader peptidase family (Peptidase_A24)

(140-247)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.617	90.785	0.423
pilD	Acinetobacter baumannii D1279779	46.617	90.785	0.423

Detailed information NTG0774533