Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PFY08_RS20920 Genome accession   NZ_CP115856
Coordinates   4093813..4094856 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus strain PL22-16A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4088813..4099856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFY08_RS20875 (PFY08_20875) - 4089247..4089447 (-) 201 WP_000106083.1 YqzE family protein -
  PFY08_RS20880 (PFY08_20880) - 4089486..4089983 (-) 498 WP_000836659.1 shikimate kinase -
  PFY08_RS20885 (PFY08_20885) - 4090098..4090748 (-) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  PFY08_RS20890 (PFY08_20890) comGG 4090923..4091294 (-) 372 WP_001231316.1 competence type IV pilus minor pilin ComGG -
  PFY08_RS20895 (PFY08_20895) comGF 4091291..4091761 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  PFY08_RS20900 (PFY08_20900) - 4091731..4092033 (-) 303 WP_000829468.1 hypothetical protein -
  PFY08_RS20905 (PFY08_20905) comGD 4092026..4092481 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  PFY08_RS20910 (PFY08_20910) comGC 4092478..4092777 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  PFY08_RS20915 (PFY08_20915) comGB 4092789..4093820 (-) 1032 WP_000475187.1 competence type IV pilus assembly protein ComGB -
  PFY08_RS20920 (PFY08_20920) comGA 4093813..4094856 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  PFY08_RS20925 (PFY08_20925) - 4095062..4095757 (+) 696 WP_000434125.1 helix-turn-helix transcriptional regulator -
  PFY08_RS20930 (PFY08_20930) - 4095883..4096125 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  PFY08_RS20935 (PFY08_20935) - 4096233..4097627 (+) 1395 WP_001094345.1 L-cystine transporter -
  PFY08_RS20940 (PFY08_20940) - 4097706..4098098 (-) 393 WP_000875394.1 hypothetical protein -
  PFY08_RS20945 (PFY08_20945) - 4098186..4098401 (-) 216 WP_001008327.1 DUF3912 family protein -
  PFY08_RS20950 (PFY08_20950) - 4098654..4098965 (+) 312 WP_001093239.1 hypothetical protein -
  PFY08_RS20955 (PFY08_20955) - 4099000..4099488 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=774007 PFY08_RS20920 WP_001013170.1 4093813..4094856(-) (comGA) [Bacillus cereus strain PL22-16A]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=774007 PFY08_RS20920 WP_001013170.1 4093813..4094856(-) (comGA) [Bacillus cereus strain PL22-16A]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372