Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PAK23_RS04775 Genome accession   NZ_CP115817
Coordinates   1042311..1043903 (-) Length   530 a.a.
NCBI ID   WP_276489277.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain GD22SC3150TT     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1037311..1048903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAK23_RS04745 - 1037361..1038077 (-) 717 WP_143498150.1 PilW family protein -
  PAK23_RS04750 pilV 1038080..1038619 (-) 540 WP_276488542.1 type IV pilus modification protein PilV -
  PAK23_RS04755 - 1038616..1039149 (-) 534 WP_276488543.1 GspH/FimT family pseudopilin -
  PAK23_RS04760 - 1039265..1039768 (-) 504 WP_276488544.1 GspH/FimT family pseudopilin -
  PAK23_RS04765 - 1039892..1040956 (+) 1065 WP_276488545.1 FAD-dependent oxidoreductase -
  PAK23_RS04770 pilR 1040968..1042308 (-) 1341 WP_276488546.1 sigma-54 dependent transcriptional regulator Regulator
  PAK23_RS04775 pilS 1042311..1043903 (-) 1593 WP_276489277.1 ATP-binding protein Regulator
  PAK23_RS04780 - 1043893..1044129 (-) 237 WP_276488547.1 PP0621 family protein -
  PAK23_RS04785 - 1044327..1045319 (-) 993 WP_276488548.1 outer membrane protein assembly factor BamD -
  PAK23_RS04790 rluD 1045458..1046432 (+) 975 WP_276488549.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PAK23_RS04795 pgeF 1046429..1047160 (+) 732 WP_276488550.1 peptidoglycan editing factor PgeF -
  PAK23_RS04800 - 1047261..1047863 (+) 603 WP_276488551.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58979.06 Da        Isoelectric Point: 8.1895

>NTDB_id=773806 PAK23_RS04775 WP_276489277.1 1042311..1043903(-) (pilS) [Pseudomonas mendocina strain GD22SC3150TT]
MIADTSVQNSKQGQRILRLYHFYRLSIGLALILLVSSNLDEQLLDVVHIELFRTGSWVYLILNIFIAVLVQKPKHLVQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAMALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVASLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRGELSGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQVDGPVLQPSFISLQRGDQQHTLVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPAD
RQIHLETQGGSLQTRMDPHQLTQVLTNLVQNGLRYSAKQHKIGQVWLRLFRDENSDLPVLEILDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQGNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=773806 PAK23_RS04775 WP_276489277.1 1042311..1043903(-) (pilS) [Pseudomonas mendocina strain GD22SC3150TT]
GTGATCGCTGACACCAGCGTACAGAACAGCAAACAAGGGCAGCGCATCCTCAGGCTCTACCACTTCTATCGCCTGAGCAT
CGGCCTGGCGCTGATCCTGCTAGTTTCCAGCAACCTCGACGAGCAGTTGCTGGATGTCGTGCATATCGAACTGTTTCGCA
CCGGCAGTTGGGTTTACCTGATCCTCAACATCTTCATCGCCGTGCTGGTGCAAAAGCCCAAGCACCTGGTGCAGCTGTTC
AGCCTGGCGCTGGTCGACGTGATCTTTCTCTCGGCCCTGTTCTACGCAGCCGGCGGCACGCCCAGCGGCATCGGCAACCT
GCTGGTGGTCGCCGTGGCCATCGCCAATATCCTGCTGCGCGGCCGCATCGGCCTGCTGATCGCCGCCATTGCCGCGATGG
CGCTGATCTACCTGACCTTCTACCTCAGCCTCAGCCGCCCCGCTGCTGCCGCACAATACGTCCAGGCCGGTGCCCTCGGC
GCGCTGTGCTTCGCCGGCGCCCTGTTCATCCAGGGCATCACCCGCCGCCTGCAGGTCAGCGAAAGCCTGGCCGAACAACG
CGCAGCGGATGTCGCCAGCCTGGAAGAACTCAACGAGCAGATCCTGCAGCGCATGCGCACCGGCATTCTGGTCCTCGACA
CCCAACATCGCGTACTGCTGGCCAACCAGGGCGCGACGCAGCTGCTCGGGCGAGGTGAACTCAGCGGCAAGATCATCGAC
CCGCACTGCCCAGAACTGGTCAAACGCCTGCAACAGTGGCTGCATAATCCGACCCTACGCCCGGACAGCCTGCAGGCGCA
GGTCGACGGCCCGGTACTGCAGCCCAGCTTCATTTCCCTGCAACGCGGCGATCAGCAGCACACCCTGGTGTTTCTCGATG
ACATCTCGCAGATCGCCCAGCAGGCGCAACAACTCAAGCTGGCCTCGCTCGGCCGCCTGACCGCCGGTATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCCATCAGCCATGCCGCGCAGCTGCTGCAGGAGTCGGAAGACCTGCAAGGCCCGGATCAACG
CCTGGCGCAGATCATTCAGGATCACTCCAAGCGCATGAACCTGGTGATCGAGAACGTTCTGCAACTGTCGCGCAGGCGCC
AGGCCGAACCGCAACTGCTCGACCTGAAGTACTGGCTGCACCGCTTCGCCAGCGAGTTTCGCAGCTCGGCACCGGCTGAC
CGACAGATCCACCTGGAGACCCAGGGCGGCAGCCTGCAGACACGCATGGACCCGCACCAGTTGACCCAGGTGCTGACCAA
TCTCGTCCAGAACGGCCTGCGCTATAGTGCCAAGCAGCACAAGATCGGTCAGGTCTGGCTGCGCCTTTTCCGTGACGAGA
ACAGCGACCTGCCGGTGCTAGAAATCCTCGACGACGGCCCCGGCGTGCCGCCCGAGCAGGAGCAGCACATCTTCGAGCCC
TTCTACACCACCGAGAACAAGGGCACCGGCCTCGGCCTGTATATTTCTCGCGAGCTGTGCCAGGGCAACCAGGCCCGCCT
CGACTATAAACCTCGTGAAGGTGGCGGCAGTTGCTTCCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

PAS domain (PAS_8)

(199-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.858

98.491

0.658