Detailed information NTG0773125

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PF550_RS01765
• Genome accession: NZ_CP115641
• Coordinates: 358772..359632 (-)
• Length: 286 a.a.
• NCBI ID: WP_001152277.1
• Uniprot ID: A0A009HWK1
• Organism: Acinetobacter baumannii strain 2022CK-00251
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 353772..364632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF550_RS01740 (PF550_01740)	-	354663..355973 (+)	1311	WP_000753281.1	tetratricopeptide repeat protein	-
PF550_RS01745 (PF550_01745)	-	356050..356376 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
PF550_RS01750 (PF550_01750)	rlmB	356478..357227 (+)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
PF550_RS01755 (PF550_01755)	-	357263..358177 (+)	915	WP_024432290.1	DMT family transporter	-
PF550_RS01760 (PF550_01760)	coaE	358174..358770 (-)	597	WP_000874125.1	dephospho-CoA kinase	-
PF550_RS01765 (PF550_01765)	pilD	358772..359632 (-)	861	WP_001152277.1	A24 family peptidase	Machinery gene
PF550_RS01770 (PF550_01770)	pilC	359632..360858 (-)	1227	WP_000279218.1	type II secretion system F family protein	Machinery gene
PF550_RS01775 (PF550_01775)	pilB	360888..362600 (-)	1713	WP_001274988.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PF550_RS01780 (PF550_01780)	tpiA	362891..363685 (+)	795	WP_000016942.1	triose-phosphate isomerase	-
PF550_RS01785 (PF550_01785)	secG	363698..364027 (+)	330	WP_000555570.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32038.43 Da
• Isoelectric Point: 7.1041

>NTDB_id=773125 PF550_RS01765 WP_001152277.1 358772..359632(-) (pilD) [Acinetobacter baumannii strain 2022CK-00251]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVIVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=773125 PF550_RS01765 WP_001152277.1 358772..359632(-) (pilD) [Acinetobacter baumannii strain 2022CK-00251]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
TATTCGCTATCCAGCCATCGAACTACTCACTATGTTATGTTCATTAGTAGTCGTCATAGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGAGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: A0A009HWK1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	98.951	100	0.99
pilD	Acinetobacter nosocomialis M2	98.601	100	0.986
pilD	Vibrio cholerae strain A1552	54.044	95.105	0.514
pilD	Vibrio campbellii strain DS40M4	52.768	94.755	0.5
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441