Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PF547_RS01475 Genome accession   NZ_CP115629
Coordinates   313150..313890 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain 2022CK-00337     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308150..318890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF547_RS01460 (PF547_01460) ponA 308734..311289 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  PF547_RS01465 (PF547_01465) - 311452..312512 (+) 1061 Protein_278 pilus assembly protein PilM -
  PF547_RS01470 (PF547_01470) pilN 312512..313153 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  PF547_RS01475 (PF547_01475) pilO 313150..313890 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  PF547_RS01480 (PF547_01480) pilP 313901..314428 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  PF547_RS01485 (PF547_01485) pilQ 314496..316661 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  PF547_RS01490 (PF547_01490) aroK 316673..317242 (+) 570 WP_000643712.1 shikimate kinase AroK -
  PF547_RS01495 (PF547_01495) aroB 317271..318353 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=772914 PF547_RS01475 WP_000076101.1 313150..313890(+) (pilO) [Acinetobacter baumannii strain 2022CK-00337]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=772914 PF547_RS01475 WP_000076101.1 313150..313890(+) (pilO) [Acinetobacter baumannii strain 2022CK-00337]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683