Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QRO24_RS02160 Genome accession   NZ_CP127829
Coordinates   458736..459296 (-) Length   186 a.a.
NCBI ID   WP_039150607.1    Uniprot ID   -
Organism   Gallibacterium anatis strain OH/Ck/12965/20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453736..464296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRO24_RS02140 (QRO24_02135) - 454442..455062 (-) 621 WP_039081190.1 tetratricopeptide repeat protein -
  QRO24_RS02145 (QRO24_02140) hisS 455074..456357 (-) 1284 WP_409025969.1 histidine--tRNA ligase -
  QRO24_RS02150 (QRO24_02145) ispG 456379..457494 (-) 1116 WP_013745263.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QRO24_RS02155 (QRO24_02150) - 457519..458592 (-) 1074 WP_039096536.1 RodZ domain-containing protein -
  QRO24_RS02160 (QRO24_02155) pilF 458736..459296 (-) 561 WP_039150607.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QRO24_RS02165 (QRO24_02160) - 459317..460435 (-) 1119 WP_013745260.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QRO24_RS02170 (QRO24_02165) folD 460753..461616 (-) 864 WP_013745259.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  QRO24_RS02185 (QRO24_02180) - 462074..462352 (-) 279 WP_018347058.1 type II toxin-antitoxin system RelE/ParE family toxin -
  QRO24_RS02190 (QRO24_02185) - 462349..462528 (-) 180 WP_013745256.1 hypothetical protein -
  QRO24_RS02195 (QRO24_02190) udk 462734..463390 (+) 657 WP_013745255.1 uridine kinase -
  QRO24_RS02200 (QRO24_02195) dcd 463406..463987 (+) 582 WP_013745254.1 dCTP deaminase -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 20951.92 Da        Isoelectric Point: 9.4938

>NTDB_id=772838 QRO24_RS02160 WP_039150607.1 458736..459296(-) (pilF) [Gallibacterium anatis strain OH/Ck/12965/20]
MNKTMIKLKFFILLSSLFTLFACSHSVSNKPLSANAAAAKTRVELGLAYLSQGNPALAKANLDKALQHTPNDYLPYLGLA
YFYQHTQQLKLAGEYYRKALSLSPENGDVLNNYATYLCSQRQFDTAFNFFEQALNSKAYYHFADTYENIAICAVLAGDQN
KTEQALTQLEKYSVGKAKNLRQRLHK

Nucleotide


Download         Length: 561 bp        

>NTDB_id=772838 QRO24_RS02160 WP_039150607.1 458736..459296(-) (pilF) [Gallibacterium anatis strain OH/Ck/12965/20]
ATGAACAAAACAATGATTAAACTGAAATTTTTTATTCTGCTTTCCTCACTGTTTACGCTTTTTGCCTGTAGCCATTCAGT
TAGCAATAAACCACTTTCCGCTAATGCTGCTGCGGCAAAAACGCGTGTTGAATTAGGATTGGCTTATTTGTCGCAAGGCA
ATCCGGCATTGGCAAAAGCCAATTTAGATAAAGCGTTGCAACATACACCGAATGATTATTTGCCTTATCTAGGTTTGGCT
TATTTTTATCAACATACTCAACAACTCAAACTTGCCGGCGAATATTATCGAAAAGCACTCTCTCTTTCGCCCGAGAATGG
CGATGTATTGAATAATTATGCAACTTACCTCTGCTCTCAACGCCAATTTGATACGGCATTTAACTTTTTTGAACAAGCAT
TAAACAGCAAAGCCTATTATCATTTTGCCGATACTTATGAAAACATTGCGATTTGCGCTGTTTTAGCCGGTGATCAAAAC
AAAACTGAACAAGCTTTAACCCAGCTGGAGAAATATTCTGTCGGTAAAGCGAAAAATTTGCGGCAACGTTTGCATAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(96-126)

Tetratricopeptide repeat (TPR_8)

(40-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

44.262

98.387

0.435
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.988

89.247

0.419