Detailed information NTG0772202

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: PDI54_RS00090
• Genome accession: NZ_CP115472
• Coordinates: 23516..24865 (+)
• Length: 449 a.a.
• NCBI ID: WP_000815646.1
• Uniprot ID: Q9XB96
• Organism: Staphylococcus aureus strain AF4001
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	23516..44856	23516..24865	within	0

Gene organization within MGE regions

Location: 23516..44856

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PDI54_RS00090 (PDI54_00090)	ccrA	23516..24865 (+)	1350	WP_000815646.1	recombinase family protein	Machinery gene
PDI54_RS00095 (PDI54_00095)	ccrB	24887..26515 (+)	1629	WP_001186602.1	recombinase family protein	Machinery gene
PDI54_RS00100 (PDI54_00100)	-	27033..27383 (+)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
PDI54_RS00105 (PDI54_00105)	-	27470..27781 (+)	312	WP_000700853.1	DUF960 family protein	-
PDI54_RS00110 (PDI54_00110)	-	27799..28305 (+)	507	WP_001092169.1	DUF1643 domain-containing protein	-
PDI54_RS00115 (PDI54_00115)	-	28326..28643 (+)	318	WP_000659050.1	JAB domain-containing protein	-
PDI54_RS00120 (PDI54_00120)	-	28762..29847 (+)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
PDI54_RS00125 (PDI54_00125)	-	29844..31736 (+)	1893	WP_001557544.1	site-specific integrase	-
PDI54_RS00130 (PDI54_00130)	-	31743..32120 (+)	378	WP_000361059.1	DUF6262 family protein	-
PDI54_RS00135 (PDI54_00135)	-	32271..33053 (+)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
PDI54_RS00140 (PDI54_00140)	erm(A)	33179..33910 (-)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
PDI54_RS14050	-	33968..34027 (-)	60	WP_010959190.1	erythromycin resistance leader peptide	-
PDI54_RS00145 (PDI54_00145)	-	34419..35081 (+)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
PDI54_RS00150 (PDI54_00150)	-	35426..36064 (-)	639	Protein_31	sulfite exporter TauE/SafE family protein	-
PDI54_RS00155 (PDI54_00155)	cstR	36064..36324 (-)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
PDI54_RS00160 (PDI54_00160)	-	36460..37524 (+)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
PDI54_RS00165 (PDI54_00165)	cstB	37557..38891 (+)	1335	Protein_34	persulfide dioxygenase-sulfurtransferase CstB	-
PDI54_RS00170 (PDI54_00170)	-	39005..40135 (-)	1131	WP_010922808.1	ROK family transcriptional regulator	-
PDI54_RS00175 (PDI54_00175)	psm-mec	40469..40537 (+)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
PDI54_RS00180 (PDI54_00180)	mecI	40622..40993 (-)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
PDI54_RS00185 (PDI54_00185)	mecR1	40993..42750 (-)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
PDI54_RS00190 (PDI54_00190)	mecA	42847..44856 (+)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52607.20 Da
• Isoelectric Point: 9.9585

>NTDB_id=772202 PDI54_RS00090 WP_000815646.1 23516..24865(+) (ccrA) [Staphylococcus aureus strain AF4001]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=772202 PDI54_RS00090 WP_000815646.1 23516..24865(+) (ccrA) [Staphylococcus aureus strain AF4001]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-322)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9XB96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	100	100	1
ccrA	Staphylococcus aureus COL	74.833	100	0.748