Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PCP67_RS08555 Genome accession   NZ_CP115289
Coordinates   1786932..1788524 (+) Length   530 a.a.
NCBI ID   WP_074222809.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F004     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1781932..1793524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP67_RS08535 (PCP67_08525) pgeF 1783682..1784410 (-) 729 WP_015503650.1 peptidoglycan editing factor PgeF -
  PCP67_RS08540 (PCP67_08530) rluD 1784407..1785369 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PCP67_RS08545 (PCP67_08535) - 1785515..1786540 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PCP67_RS08550 (PCP67_08540) - 1786697..1786942 (+) 246 WP_003102591.1 PP0621 family protein -
  PCP67_RS08555 (PCP67_08545) pilS 1786932..1788524 (+) 1593 WP_074222809.1 two-component system sensor histidine kinase PilS Regulator
  PCP67_RS08560 (PCP67_08550) pilR 1788539..1789876 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  PCP67_RS08565 (PCP67_08555) thiO 1789922..1791016 (-) 1095 WP_031756968.1 glycine oxidase ThiO -
  PCP67_RS08570 (PCP67_08560) - 1791151..1791660 (+) 510 WP_034014851.1 GspH/FimT family pseudopilin -
  PCP67_RS08575 (PCP67_08565) - 1791766..1792272 (+) 507 WP_003110423.1 GspH/FimT family pseudopilin -
  PCP67_RS08580 (PCP67_08570) pilV 1792263..1792820 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59002.14 Da        Isoelectric Point: 6.5648

>NTDB_id=771402 PCP67_RS08555 WP_074222809.1 1786932..1788524(+) (pilS) [Pseudomonas aeruginosa strain F004]
MRAERLRLSDEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=771402 PCP67_RS08555 WP_074222809.1 1786932..1788524(+) (pilS) [Pseudomonas aeruginosa strain F004]
GTGCGCGCTGAACGGCTACGGCTGAGCGACGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
GCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGACAAATTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.434

100

0.994