Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilD   Type   Machinery gene
Locus tag   PCP32_RS14770 Genome accession   NZ_CP115285
Coordinates   3170438..3171310 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3165438..3176310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP32_RS14745 (PCP32_14740) nadC 3165771..3166619 (+) 849 WP_058152957.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP32_RS14755 (PCP32_14750) - 3166822..3167286 (-) 465 WP_031630343.1 pilin -
  PCP32_RS14760 (PCP32_14755) pilB 3167516..3169216 (+) 1701 WP_058152958.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP32_RS14765 (PCP32_14760) pilC 3169220..3170437 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PCP32_RS14770 (PCP32_14765) pilD 3170438..3171310 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP32_RS14775 (PCP32_14770) coaE 3171307..3171918 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP32_RS14780 (PCP32_14775) yacG 3171915..3172115 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP32_RS14785 (PCP32_14780) - 3172152..3172361 (-) 210 WP_003094660.1 hypothetical protein -
  PCP32_RS14790 (PCP32_14785) - 3172467..3173156 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PCP32_RS14795 (PCP32_14790) - 3173153..3173623 (-) 471 WP_003094664.1 hypothetical protein -
  PCP32_RS14800 (PCP32_14795) - 3173620..3174045 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PCP32_RS14805 (PCP32_14800) - 3174178..3174807 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PCP32_RS14810 (PCP32_14805) - 3174804..3175253 (+) 450 WP_058152959.1 MOSC domain-containing protein -
  PCP32_RS14815 (PCP32_14810) - 3175279..3175449 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence


Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=771303 PCP32_RS14770 WP_003125204.1 3170438..3171310(+) (pilD) [Pseudomonas aeruginosa strain F007]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=771303 PCP32_RS14770 WP_003125204.1 3170438..3171310(+) (pilD) [Pseudomonas aeruginosa strain F007]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538

  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517

  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493

  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493

  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472