Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QP019_RS02935 Genome accession   NZ_CP126978
Coordinates   554858..555418 (-) Length   186 a.a.
NCBI ID   WP_013745261.1    Uniprot ID   -
Organism   Gallibacterium anatis strain YJ922     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549858..560418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP019_RS02915 (QP019_02915) - 550564..551184 (-) 621 WP_039081190.1 tetratricopeptide repeat protein -
  QP019_RS02920 (QP019_02920) hisS 551196..552479 (-) 1284 WP_285096635.1 histidine--tRNA ligase -
  QP019_RS02925 (QP019_02925) ispG 552501..553616 (-) 1116 WP_018347055.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QP019_RS02930 (QP019_02930) - 553641..554714 (-) 1074 WP_285096636.1 RodZ domain-containing protein -
  QP019_RS02935 (QP019_02935) pilF 554858..555418 (-) 561 WP_013745261.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QP019_RS02940 (QP019_02940) - 555439..556557 (-) 1119 WP_013745260.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QP019_RS02945 (QP019_02945) folD 556876..557739 (-) 864 WP_285096637.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  QP019_RS02960 (QP019_02960) - 558212..558490 (-) 279 WP_018347058.1 hypothetical protein -
  QP019_RS02965 (QP019_02965) - 558487..558666 (-) 180 WP_013745256.1 hypothetical protein -
  QP019_RS02970 (QP019_02970) udk 558872..559528 (+) 657 WP_285096638.1 uridine kinase -
  QP019_RS02975 (QP019_02975) dcd 559544..560125 (+) 582 WP_013745254.1 dCTP deaminase -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 20953.95 Da        Isoelectric Point: 9.3348

>NTDB_id=769717 QP019_RS02935 WP_013745261.1 554858..555418(-) (pilF) [Gallibacterium anatis strain YJ922]
MNKTMIKLKFFILLSSLFTLFACSHSVSNKPLSANAAAAKTRVELGLAYLSQGNPALAKANLDKALQHTPNDYLPYLGLA
YFYQHTQQLKLAGEYYQKALSLSPENGDVLNNYATYLCSQRQFDMAFNFFEQALNSKAYYHFADTYENIAICAVLAGDQN
KTEQALTQLEKYSVGKAKNLRQRLHK

Nucleotide


Download         Length: 561 bp        

>NTDB_id=769717 QP019_RS02935 WP_013745261.1 554858..555418(-) (pilF) [Gallibacterium anatis strain YJ922]
ATGAACAAAACAATGATTAAACTGAAATTTTTTATTCTGCTTTCCTCACTGTTTACGCTTTTTGCCTGTAGCCATTCAGT
TAGCAATAAACCACTTTCCGCTAATGCCGCTGCGGCAAAAACGCGTGTTGAATTAGGATTGGCTTATTTGTCGCAAGGTA
ATCCGGCATTGGCAAAAGCCAATTTAGATAAAGCGTTGCAACATACACCGAATGATTATTTGCCTTATCTAGGTTTGGCT
TATTTTTATCAACATACCCAACAACTCAAACTTGCCGGCGAATATTATCAAAAAGCGCTCTCTCTTTCGCCCGAGAATGG
CGATGTATTGAATAATTATGCAACTTACCTCTGCTCTCAACGCCAATTTGATATGGCATTTAACTTTTTTGAACAAGCAT
TAAACAGCAAAGCCTATTATCATTTTGCCGATACTTATGAAAACATTGCGATTTGCGCTGTTTTAGCCGGTGATCAAAAC
AAAACTGAACAAGCTTTAACCCAACTGGAGAAATATTCTGTCGGTAAAGCGAAAAATTTGCGGCAACGTTTGCATAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(40-68)

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

44.809

98.387

0.441
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.988

89.247

0.419