Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QP018_RS02075 Genome accession   NZ_CP126975
Coordinates   446589..447149 (-) Length   186 a.a.
NCBI ID   WP_285091178.1    Uniprot ID   A0AAX3XE25
Organism   Gallibacterium anatis strain BJF12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441589..452149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP018_RS02055 (QP018_02055) - 442295..442915 (-) 621 WP_039081190.1 tetratricopeptide repeat protein -
  QP018_RS02060 (QP018_02060) hisS 442927..444210 (-) 1284 WP_013745264.1 histidine--tRNA ligase -
  QP018_RS02065 (QP018_02065) ispG 444232..445347 (-) 1116 WP_285091180.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QP018_RS02070 (QP018_02070) - 445372..446445 (-) 1074 WP_285091179.1 RodZ domain-containing protein -
  QP018_RS02075 (QP018_02075) pilF 446589..447149 (-) 561 WP_285091178.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QP018_RS02080 (QP018_02080) - 447170..448288 (-) 1119 WP_039083483.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QP018_RS02085 (QP018_02085) folD 448606..449469 (-) 864 WP_013745259.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  QP018_RS02100 (QP018_02100) - 449897..450892 (-) 996 WP_039136989.1 site-specific integrase -
  QP018_RS02105 (QP018_02105) - 451338..451835 (-) 498 WP_285091175.1 hypothetical protein -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21011.99 Da        Isoelectric Point: 9.1368

>NTDB_id=769607 QP018_RS02075 WP_285091178.1 446589..447149(-) (pilF) [Gallibacterium anatis strain BJF12]
MNKTMIKLKFFILLSSLFTLFACSHSVSNKPLSANAAAAKTRVELGLAYLSQGNPALAKANLDKALQHTPNDYLPYLGLA
YFYQHTQQLKLAGEYYQKALSLSPENGDVLNNYATYLCSQRQFDMAFNFFEQALNSKAYYHFADTYENIAICAVLAGDQN
KTEQALTQLEKYSVDKAKNLRQRLHK

Nucleotide


Download         Length: 561 bp        

>NTDB_id=769607 QP018_RS02075 WP_285091178.1 446589..447149(-) (pilF) [Gallibacterium anatis strain BJF12]
ATGAACAAAACAATGATTAAACTGAAATTTTTTATTCTGCTTTCCTCACTGTTTACGCTTTTTGCCTGTAGCCATTCAGT
TAGCAATAAACCACTTTCCGCTAATGCTGCTGCGGCAAAAACGCGTGTTGAATTAGGATTGGCTTATTTGTCGCAAGGCA
ATCCGGCATTGGCAAAAGCCAATTTAGATAAAGCGTTGCAACATACACCGAATGATTATTTGCCTTATCTAGGTTTGGCT
TATTTTTATCAACATACCCAACAACTCAAACTTGCCGGCGAATATTATCAAAAAGCGCTCTCTCTTTCGCCCGAGAATGG
CGATGTATTGAATAATTATGCAACTTACCTCTGCTCTCAACGCCAATTTGATATGGCATTTAACTTTTTTGAACAAGCAT
TAAACAGCAAAGCCTATTATCATTTTGCCGATACTTATGAAAACATTGCGATTTGTGCTGTTTTAGCCGGTGATCAAAAC
AAAACTGAACAAGCTTTAACCCAACTGGAGAAATATTCTGTCGATAAAGCGAAAAATTTGCGGCAACGTTTGCATAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(40-68)

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AAX3XE25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

44.809

98.387

0.441
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.988

89.247

0.419