Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O8413_RS13420 Genome accession   NZ_CP115188
Coordinates   2872531..2873637 (+) Length   368 a.a.
NCBI ID   WP_004724258.1    Uniprot ID   -
Organism   Vibrio furnissii strain MT14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2867531..2878637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8413_RS13385 (O8413_13385) - 2867883..2868482 (-) 600 WP_055467258.1 XTP/dITP diphosphatase -
  O8413_RS13390 (O8413_13390) - 2868506..2868937 (-) 432 WP_283512666.1 DUF4426 domain-containing protein -
  O8413_RS13395 (O8413_13395) yggU 2868994..2869284 (-) 291 WP_004724263.1 DUF167 family protein YggU -
  O8413_RS13400 (O8413_13400) - 2869284..2869841 (-) 558 WP_004724262.1 YggT family protein -
  O8413_RS13405 (O8413_13405) proC 2869893..2870711 (-) 819 WP_004724261.1 pyrroline-5-carboxylate reductase -
  O8413_RS13410 (O8413_13410) - 2870749..2871456 (-) 708 WP_014205824.1 YggS family pyridoxal phosphate-dependent enzyme -
  O8413_RS13415 (O8413_13415) pilT 2871482..2872519 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  O8413_RS13420 (O8413_13420) pilU 2872531..2873637 (+) 1107 WP_004724258.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O8413_RS13425 (O8413_13425) ruvX 2873734..2874159 (-) 426 WP_283512667.1 Holliday junction resolvase RuvX -
  O8413_RS13430 (O8413_13430) - 2874222..2874785 (-) 564 WP_004724256.1 YqgE/AlgH family protein -
  O8413_RS13435 (O8413_13435) gshB 2874824..2875780 (-) 957 WP_004724255.1 glutathione synthase -
  O8413_RS13440 (O8413_13440) rsmE 2875793..2876524 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  O8413_RS13445 (O8413_13445) - 2876643..2877344 (-) 702 WP_208549353.1 endonuclease -
  O8413_RS13450 (O8413_13450) - 2877483..2877983 (-) 501 WP_004724252.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41357.36 Da        Isoelectric Point: 6.4509

>NTDB_id=768823 O8413_RS13420 WP_004724258.1 2872531..2873637(+) (pilU) [Vibrio furnissii strain MT14]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVRTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTMAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKTQRGDVTSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=768823 O8413_RS13420 WP_004724258.1 2872531..2873637(+) (pilU) [Vibrio furnissii strain MT14]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTGTATATCACTGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAACTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCAGGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGATTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGCATTGAAACGCAAATTCCCACGTTTGATGAACT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGTTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCGATGACCGGGTATCGCAATATGCATCGCACCGGGCATATTCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGCGAAGTGGGATTGGACACGGAAAGTTATGAAGTCGCACT
GAAGAACTCGCTGCGTCAGGCGCCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCAATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCACTTGAGCGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGGGTTGTCGGTCAGCAACTGAT
CCGCGATAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GCGGCGAGCTGCATGAGTTGAAAAACACCATGGCGAAATCAAAAGAAGTCGGCATGCAGACGTTTGATCAGGCGCTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGCGAGCAAGATGCGCTGCACAGTGCGGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGACGCAGCGGGGCGATGTGACCAGCAGCGGGTCGCTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.87

100

0.859
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.077

88.315

0.38
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361