Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N8M53_RS01045 Genome accession   NZ_CP114588
Coordinates   214847..216592 (+) Length   581 a.a.
NCBI ID   WP_420066593.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 209847..221592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8M53_RS01025 (N8M53_01025) - 212249..213145 (+) 897 WP_269579185.1 hypothetical protein -
  N8M53_RS01030 (N8M53_01030) - 213142..213723 (+) 582 WP_269579186.1 PilN domain-containing protein -
  N8M53_RS01035 (N8M53_01035) - 213720..214301 (+) 582 WP_269579187.1 type 4a pilus biogenesis protein PilO -
  N8M53_RS01040 (N8M53_01040) - 214323..214832 (+) 510 WP_269579188.1 pilus assembly protein PilP -
  N8M53_RS01045 (N8M53_01045) pilQ 214847..216592 (+) 1746 WP_420066593.1 type IV pilus secretin PilQ Machinery gene
  N8M53_RS01050 (N8M53_01050) aroK 216737..217255 (+) 519 WP_025739049.1 shikimate kinase AroK -
  N8M53_RS01055 (N8M53_01055) aroB 217275..218354 (+) 1080 WP_269579189.1 3-dehydroquinate synthase -
  N8M53_RS01060 (N8M53_01060) - 218366..219970 (+) 1605 WP_269579190.1 AAA family ATPase -
  N8M53_RS01065 (N8M53_01065) dam 220043..220873 (+) 831 WP_269579191.1 adenine-specific DNA-methyltransferase -
  N8M53_RS01070 (N8M53_01070) - 220905..221093 (-) 189 WP_269579192.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62748.88 Da        Isoelectric Point: 5.0645

>NTDB_id=766644 N8M53_RS01045 WP_420066593.1 214847..216592(+) (pilQ) [Salinivibrio kushneri strain TGB11]
MKASRVRSTFGSGLAQAAIITVFACGMVWSAVVGAAEAVTLVQAQLTSSTQQETQLRLRFDSAAVMADFRQTSPQQLMIR
LPQVVVTPDLLGRMKGQETGLPVNAVHITQEAGYTKIEIHAEQAMTFDYQQQHQQLIITLRDSDPVTAQAPAPEAPISIN
FQDIPVRAVLQLIAEQNQFNLVTTDSVQGNITMRIDGVPWQDVLDVILRVKGLDKRQRGDVMLVAPRRELMAQEKQDLEM
AQQADALLPLSSDVITVNYAKAGDVAALIRGQGQGVSLLSARGAVSLDSRTNAIIVRDIEKNIERIRTLVSHLDVPVKQV
EIEARIVTVEQGSVDELGVRWGLLNRNGSTTVGPSVEGNLMWDSNVTGRGPESLNDAATDGANIGDFLSVNLAAANPSAS
SMALQVAKLGNDLLLDLELSALESERKAEVVSSPRLITTNKKAAYIEQGTELPYLEASSSGAVAVSFKKAVLSLSVTPQI
TPNNTLVLDLKLTQDMPEKAVVAGKGEAMSISTKRINTQVLAENGETVVLGGIFQQTMINKETQVPLLGDIPLVGNLFKH
QLKDHAKRELLIFVTPKIVTD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=766644 N8M53_RS01045 WP_420066593.1 214847..216592(+) (pilQ) [Salinivibrio kushneri strain TGB11]
ATGAAAGCAAGCAGGGTAAGGAGCACATTCGGATCAGGACTGGCGCAAGCCGCAATTATCACTGTTTTCGCTTGTGGGAT
GGTATGGAGTGCTGTAGTGGGGGCCGCTGAGGCGGTGACCTTGGTACAGGCGCAACTCACGTCTTCTACTCAACAAGAAA
CGCAGCTCCGCTTGCGTTTTGACAGTGCCGCGGTGATGGCGGATTTTCGCCAGACGAGCCCTCAGCAGCTAATGATTAGG
CTACCTCAGGTGGTCGTCACGCCAGATTTGCTCGGGCGGATGAAAGGGCAAGAGACGGGTCTGCCTGTGAACGCCGTTCA
CATCACTCAGGAAGCAGGCTATACCAAGATAGAGATTCACGCCGAGCAAGCGATGACGTTTGATTACCAGCAACAGCATC
AGCAATTAATTATCACCTTGCGCGATTCTGATCCGGTGACCGCTCAAGCCCCGGCCCCCGAGGCGCCGATTTCCATCAAT
TTCCAAGATATTCCTGTGCGGGCAGTGCTACAACTTATTGCCGAGCAAAATCAATTCAATTTGGTGACCACTGACAGTGT
GCAGGGCAATATCACCATGCGTATCGACGGGGTGCCATGGCAAGATGTGTTGGATGTGATCCTCAGGGTCAAGGGGCTAG
ACAAGCGTCAACGTGGGGATGTGATGTTAGTTGCGCCTCGTAGAGAGCTCATGGCGCAAGAAAAGCAAGATCTCGAGATG
GCGCAACAAGCAGACGCACTGTTGCCTTTGTCTTCTGATGTGATCACCGTTAACTACGCCAAAGCCGGTGACGTTGCGGC
ATTAATCCGAGGCCAGGGTCAGGGAGTGAGCTTACTGTCTGCTCGTGGCGCTGTCTCACTCGATTCGCGCACTAATGCGA
TCATCGTTCGTGATATTGAGAAGAACATCGAGCGTATCCGCACCTTGGTGAGTCACTTAGATGTGCCCGTTAAGCAAGTG
GAGATTGAGGCACGTATTGTGACGGTCGAGCAAGGCTCAGTAGACGAATTAGGTGTACGTTGGGGGCTGTTGAATCGAAA
TGGCTCAACCACGGTTGGTCCGTCTGTGGAAGGTAACTTGATGTGGGATAGCAATGTCACCGGGCGAGGCCCTGAAAGCT
TAAACGATGCGGCGACAGACGGCGCCAATATTGGTGACTTCCTCAGTGTGAACCTGGCAGCTGCTAACCCCTCCGCCTCG
AGTATGGCCTTACAAGTGGCGAAGCTCGGCAATGATCTTCTGCTCGACTTGGAGCTGTCTGCGCTTGAAAGCGAAAGGAA
AGCAGAAGTGGTATCGAGCCCTCGACTTATCACCACCAATAAAAAAGCGGCCTACATTGAACAAGGGACAGAACTGCCTT
ATCTCGAGGCTTCTTCAAGCGGTGCGGTGGCAGTGTCTTTTAAAAAAGCGGTGTTGAGTTTATCGGTGACCCCACAAATC
ACGCCCAATAATACGCTGGTCTTGGATTTAAAGTTAACCCAAGATATGCCCGAAAAAGCTGTGGTGGCCGGAAAAGGCGA
AGCGATGTCGATCAGTACCAAACGCATTAATACCCAAGTACTGGCGGAAAATGGTGAAACCGTAGTCTTAGGGGGCATCT
TTCAGCAAACCATGATCAACAAAGAGACCCAAGTGCCGCTGCTGGGGGATATTCCGTTGGTTGGTAACCTGTTTAAGCAC
CAGTTAAAAGACCATGCAAAACGCGAACTTCTGATCTTTGTGACCCCTAAAATCGTCACAGACTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(421-579)

Bacterial type II/III secretion system short domain (Secretin_N)

(254-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

49.385

97.935

0.484
  pilQ Vibrio cholerae strain A1552

49.385

97.935

0.484
  pilQ Vibrio campbellii strain DS40M4

52.662

90.534

0.477
  pilQ Legionella pneumophila strain ERS1305867

42.744

86.575

0.37
  pilQ Legionella pneumophila strain Lp02

42.545

86.575

0.368
  comQ Acinetobacter baylyi ADP1

39.219

92.599

0.363