Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H7K53_RS02495 Genome accession   NZ_AP022077
Coordinates   524504..525304 (+) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain WP4-W18-ESBL-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 519504..530304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K53_RS02470 (WP4W18E11_04710) - 520414..521712 (+) 1299 WP_000348499.1 glycosyltransferase family 1 protein -
  H7K53_RS02475 (WP4W18E11_04720) - 521737..522288 (+) 552 WP_000669564.1 phosphatase PAP2 family protein -
  H7K53_RS02480 (WP4W18E11_04730) iscX 522388..522588 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  H7K53_RS02485 (WP4W18E11_04740) ndk 522702..523133 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  H7K53_RS02490 (WP4W18E11_04750) rlmN 523256..524488 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H7K53_RS02495 (WP4W18E11_04760) pilF 524504..525304 (+) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H7K53_RS02500 (WP4W18E11_04770) - 525295..526086 (+) 792 WP_000405161.1 RodZ domain-containing protein -
  H7K53_RS02505 (WP4W18E11_04780) ispG 526108..527223 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H7K53_RS02510 (WP4W18E11_04790) hisS 527220..528512 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  H7K53_RS02515 (WP4W18E11_04800) - 528541..529239 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=76525 H7K53_RS02495 WP_001984465.1 524504..525304(+) (pilF) [Acinetobacter baumannii strain WP4-W18-ESBL-11]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=76525 H7K53_RS02495 WP_001984465.1 524504..525304(+) (pilF) [Acinetobacter baumannii strain WP4-W18-ESBL-11]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment