Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QMY08_RS12545 Genome accession   NZ_CP125889
Coordinates   2443244..2444677 (+) Length   477 a.a.
NCBI ID   WP_201520128.1    Uniprot ID   -
Organism   Escherichia coli strain EPSSH018     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2438244..2449677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY08_RS12515 (QMY08_12460) yrbG 2438802..2439779 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  QMY08_RS12520 (QMY08_12465) kdsD 2439793..2440779 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QMY08_RS12525 (QMY08_12470) kdsC 2440800..2441366 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QMY08_RS12530 (QMY08_12475) lptC 2441363..2441938 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QMY08_RS12535 (QMY08_12480) lptA 2441907..2442464 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QMY08_RS12540 (QMY08_12485) lptB 2442471..2443196 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QMY08_RS12545 (QMY08_12490) rpoN/rpoN1 2443244..2444677 (+) 1434 WP_201520128.1 RNA polymerase factor sigma-54 Machinery gene
  QMY08_RS12550 (QMY08_12495) hpf 2444700..2444987 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QMY08_RS12555 (QMY08_12500) ptsN 2445105..2445596 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QMY08_RS12560 (QMY08_12505) rapZ 2445642..2446496 (+) 855 WP_000243741.1 RNase adapter RapZ -
  QMY08_RS12565 (QMY08_12510) npr 2446493..2446765 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QMY08_RS12570 (QMY08_12515) yrbL 2446979..2447611 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  QMY08_RS12575 (QMY08_12520) mtgA 2447608..2448336 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QMY08_RS12580 (QMY08_12525) elbB 2448333..2448986 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53924.68 Da        Isoelectric Point: 4.3380

>NTDB_id=764936 QMY08_RS12545 WP_201520128.1 2443244..2444677(+) (rpoN/rpoN1) [Escherichia coli strain EPSSH018]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSSCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=764936 QMY08_RS12545 WP_201520128.1 2443244..2444677(+) (rpoN/rpoN1) [Escherichia coli strain EPSSH018]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTAGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465