Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   O2T11_RS14540 Genome accession   NZ_CP114186
Coordinates   3044400..3044993 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain PH1273     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3039400..3049993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2T11_RS14520 (O2T11_14520) aroB 3040276..3041376 (-) 1101 WP_140079228.1 3-dehydroquinate synthase -
  O2T11_RS14525 (O2T11_14525) aroK 3041408..3041926 (-) 519 WP_005381319.1 shikimate kinase AroK -
  O2T11_RS14530 (O2T11_14530) pilQ 3042115..3043863 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  O2T11_RS14535 (O2T11_14535) pilP 3043895..3044410 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  O2T11_RS14540 (O2T11_14540) pilO 3044400..3044993 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  O2T11_RS14545 (O2T11_14545) pilN 3044986..3045564 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  O2T11_RS14550 (O2T11_14550) pilM 3045548..3046564 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  O2T11_RS14555 (O2T11_14555) - 3046707..3049277 (+) 2571 WP_140079229.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=764936 O2T11_RS14540 WP_005480482.1 3044400..3044993(-) (pilO) [Vibrio parahaemolyticus strain PH1273]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=764936 O2T11_RS14540 WP_005480482.1 3044400..3044993(-) (pilO) [Vibrio parahaemolyticus strain PH1273]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726