Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OXH74_RS06240 Genome accession   NZ_CP113889
Coordinates   1362764..1363828 (-) Length   354 a.a.
NCBI ID   WP_375740507.1    Uniprot ID   -
Organism   Pseudomonas boanensis strain L337     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357764..1368828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH74_RS06215 (OXH74_06200) aroK 1358295..1358813 (-) 519 WP_375741572.1 shikimate kinase AroK -
  OXH74_RS06220 (OXH74_06205) pilQ 1358819..1360954 (-) 2136 WP_375741573.1 type IV pilus secretin PilQ Machinery gene
  OXH74_RS06225 (OXH74_06210) pilP 1361009..1361542 (-) 534 WP_375740504.1 type 4a pilus biogenesis lipoprotein PilP -
  OXH74_RS06230 (OXH74_06215) pilO 1361539..1362162 (-) 624 WP_375740505.1 type 4a pilus biogenesis protein PilO -
  OXH74_RS06235 (OXH74_06220) pilN 1362159..1362764 (-) 606 WP_375740506.1 type 4a pilus biogenesis protein PilN -
  OXH74_RS06240 (OXH74_06225) pilM 1362764..1363828 (-) 1065 WP_375740507.1 pilus assembly protein PilM Machinery gene
  OXH74_RS06245 (OXH74_06230) - 1364013..1366433 (+) 2421 WP_375740508.1 penicillin-binding protein 1A -
  OXH74_RS06250 (OXH74_06235) - 1366529..1367797 (-) 1269 WP_375740509.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37842.08 Da        Isoelectric Point: 4.4040

>NTDB_id=763346 OXH74_RS06240 WP_375740507.1 1362764..1363828(-) (pilM) [Pseudomonas boanensis strain L337]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRYKVESYAVEPLPPNAVVEKNIAELEGVGQAISRVVTKARTGVKAAAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGPSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLSDQLGGGQDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASLPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=763346 OXH74_RS06240 WP_375740507.1 1362764..1363828(-) (pilM) [Pseudomonas boanensis strain L337]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTGGAGTCCTATGCCGTCGAGCCGCTCCCGCCCAATGCTGTGGTTGAAAAGAACA
TTGCCGAGCTGGAAGGTGTCGGGCAAGCCATTTCACGTGTCGTGACCAAGGCTCGAACGGGCGTCAAGGCAGCGGCGGTT
GCGGTTGCCGGCTCAGCGGTCATCACCAAGACGATCGAGATGGAAGCGGGACTCTCCGATGACGATTTGGAGAACCAGCT
GAAGATCGAAGCCGATCAGTACATTCCTTACCCTTTGGAAGAAGTGGCAATCGACTTCGAAGTGCAGGGACCTTCCGCCC
GCAACCCGGAGCGGGTCGATGTGCTGCTGGCCGCTTGTCGTAAGGAAAACGTCGAAGTCCGCGAGGCTGCCCTCGCGCTG
GCCGGACTGACCGCCAAGGTGGTCGATGTCGAGGCGTACGCCTTGGAGCGTGCCTACGGCCTGCTCTCGGATCAGCTGGG
CGGCGGCCAGGACGAATTGACCGTTGCCGTGGTCGATATTGGCGCCACCATGACTACGCTCAGCGTCCTGCATAACGGCC
GCACCATCTATACCCGCGAGCAGCTTTTCGGTGGGCGTCAGCTGACCGAGGAAATCCAGCGCCGCTATGGGCTGTCGATG
GAGGAGGCCGGTCTTGCCAAGAAGCAGGGTGGTCTTCCAGACGACTACGACAGCGAGGTCTTGCAACCGTTCAAGGATGC
GGTGGTCCAGCAAGTTTCCCGATCCCTGCAATTCTTCTTCGCGGCGGGTCAGTACAACGATGTCGATTACATCCTGCTGG
CTGGTGGCACCGCCTCCCTCCCTGATCTGGATCGCCTGATACAGCAGAAAATCGGGACTCCGACCCTGGTCGCCAACCCC
TTTGCCGACATGGCGCTGAGCAGCAAGGTCAACGCTGGTGCCTTGGCCAGTGATGCCCCTGCGCTGATGATTGCCTGCGG
TTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

47.606

100

0.477