Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   BSU_11530 Genome accession   NC_000964
Coordinates   1229915..1231036 (+) Length   373 a.a.
NCBI ID   NP_389035.1    Uniprot ID   O31604
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination; DNA uptake   
Homologous recombination DNA binding and uptake

Function

During competence, CoiA is believed to colocalize with other proteins such as ComFA, ComGA, DprA, RecA, and SsbB at the cell pole, suggesting its involvement in the DNA uptake and recombination process.


Genomic Context

Location: 1224915..1236036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_11480 (BSU11480) yjbB 1225557..1226774 (+) 1218 NP_389030.2 putative exporter -
  BSU_11490 (BSU11490) yjbC 1226938..1227516 (+) 579 NP_389031.2 putative thiol oxidation management factor; putative acetyltransferase -
  BSU_11500 (BSU11500) spxA 1227697..1228092 (+) 396 NP_389032.1 redox-sensitive regulator -
  BSU_11510 (BSU11510) yjbE 1228135..1228791 (-) 657 NP_389033.1 putative membrane protein of unknown function -
  BSU_11515 (BSU11515) - 1228961..1229101 (+) 141 YP_009513949.1 hypothetical protein -
  BSU_11520 (BSU11520) mecA 1229068..1229724 (+) 657 NP_389034.1 adaptor protein controlling oligomerization of the AAA+ protein ClpC Regulator
  BSU_11525 (BSU11525) - 1229719..1229841 (-) 123 YP_009513950.1 hypothetical protein -
  BSU_11530 (BSU11530) coiA 1229915..1231036 (+) 1122 NP_389035.1 protein involved in establishment of DNA transport in competence Machinery gene
  BSU_11540 (BSU11540) pepF 1231083..1233095 (+) 2013 NP_389036.2 oligoendopeptidase F -
  BSU_11549 (BSU11549) yizD 1233133..1233300 (-) 168 YP_003097703.1 hypothetical protein -
  BSU_11550 (BSU11550) spxH 1233614..1234513 (-) 900 NP_389037.2 thiol management effector of SpxA degradation -
  BSU_11560 (BSU11560) yjbI 1234510..1234908 (-) 399 NP_389038.1 putative thiol management oxidoreductase component -
  BSU_11570 (BSU11570) cwlQ 1235163..1235708 (-) 546 NP_389039.1 bifunctional muramidase and murein lytic transglycosylase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 43689.70 Da        Isoelectric Point: 10.1187

>NTDB_id=123 BSU_11530 NP_389035.1 1229915..1231036(+) (coiA) [Bacillus subtilis subsp. subtilis str. 168]
MFHLLGAQQNQKLKRRRFFCPVCGGELAVKLGLQKAPHFAHKQNKSCAIDIEPESAYHLEGKRQLYVWLKTQRASPILEP
YIRTINQRPDVMARIKEHMLAVEYQCATIAPDVFQKRTEGFKQEGIIPQWIMGYSRLKRTASSFYQLSTFHWQFINASPY
RELICYCPERRSFLRLSHIIPFYTNHSYSSVQTIPIHRAGAGDLFFTEPKPSIQYSGWTKAIHRFRHKPHRFNSKETNRL
RLLFYEKRQTPFSFLPTEVFVPVRKGAVFKSPVFVWQGFLYLFMTDLGGKRAPIRFSAVLQQCKLHIHNKNIALRSECSE
ECLSEAVKQYIDFLCKKGFLRETQKEVYVLNQPAGGIHSMQDLIERDRSCFIE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=123 BSU_11530 NP_389035.1 1229915..1231036(+) (coiA) [Bacillus subtilis subsp. subtilis str. 168]
ATGTTTCACCTTCTAGGCGCTCAGCAAAACCAGAAATTAAAGCGGAGACGGTTCTTCTGTCCGGTATGCGGGGGAGAGCT
AGCTGTGAAGCTCGGACTTCAAAAAGCGCCGCATTTTGCCCATAAACAGAACAAGTCCTGCGCCATAGACATCGAGCCGG
AAAGCGCCTACCACTTAGAAGGAAAAAGACAGCTGTATGTATGGCTGAAAACGCAACGGGCTTCACCAATATTGGAGCCG
TATATAAGGACAATCAACCAAAGACCGGACGTCATGGCAAGAATCAAGGAGCATATGCTTGCTGTTGAATATCAATGCGC
CACTATCGCTCCAGATGTCTTTCAAAAGCGGACAGAGGGCTTCAAACAAGAAGGAATCATCCCGCAGTGGATTATGGGAT
ACAGCCGCTTAAAACGGACTGCTTCGTCCTTTTATCAGCTTTCAACTTTTCATTGGCAGTTTATCAATGCAAGTCCATAC
AGAGAACTGATATGTTATTGTCCTGAAAGGCGTTCTTTTCTCCGGCTCAGCCACATCATTCCATTCTACACAAACCATTC
TTATTCCTCAGTCCAAACGATCCCTATCCATCGGGCAGGTGCAGGCGATCTTTTTTTCACTGAACCGAAACCCTCAATTC
AATATTCAGGCTGGACGAAAGCGATTCACCGTTTTCGCCATAAACCCCATCGGTTTAACTCAAAGGAAACAAATCGCCTT
AGGCTGCTGTTTTATGAGAAACGACAGACGCCTTTTTCTTTTCTGCCGACTGAAGTGTTTGTTCCTGTTAGAAAAGGGGC
AGTTTTTAAGAGTCCGGTTTTTGTGTGGCAGGGGTTTCTTTATTTGTTTATGACGGATCTTGGAGGTAAACGTGCGCCAA
TTCGTTTTTCAGCCGTTTTGCAGCAGTGCAAACTGCACATTCATAATAAGAACATTGCACTCAGGTCCGAATGCAGCGAA
GAGTGTCTTTCAGAAGCGGTTAAACAGTATATTGATTTTTTGTGCAAGAAGGGATTTTTAAGGGAGACTCAGAAAGAAGT
ATATGTGCTGAATCAGCCTGCAGGAGGCATACACTCCATGCAGGATCTCATAGAAAGAGACCGCAGCTGTTTTATAGAAT
GA

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(12-363)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O31604

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Naomi Kramer et al. (2007) Multiple interactions among the competence proteins of Bacillus subtilis. Molecular Microbiology 65(2):454-64. [PMID: 17630974]