Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   M2Z51_RS01210 Genome accession   NZ_AP024967
Coordinates   263276..265480 (+) Length   734 a.a.
NCBI ID   WP_001247903.1    Uniprot ID   -
Organism   Vibrio cholerae strain V130003     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258276..270480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2Z51_RS01190 (V130003_02280) - 259357..260523 (-) 1167 WP_000213237.1 O-succinylhomoserine (thiol)-lyase -
  M2Z51_RS01195 (V130003_02290) metJ 260791..261108 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  M2Z51_RS01200 (V130003_02300) - 261218..262495 (-) 1278 WP_032477253.1 malic enzyme-like NAD(P)-binding protein -
  M2Z51_RS01205 (V130003_02310) rpmE 262803..263021 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  M2Z51_RS01210 (V130003_02320) priA 263276..265480 (+) 2205 WP_001247903.1 primosomal protein N' Machinery gene
  M2Z51_RS01215 (V130003_02330) cytR 265786..266793 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  M2Z51_RS01220 (V130003_02340) - 266965..267516 (+) 552 WP_001891621.1 SPOR domain-containing protein -
  M2Z51_RS01225 (V130003_02350) hslV 267652..268209 (+) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  M2Z51_RS01230 (V130003_02360) hslU 268275..269606 (+) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82080.42 Da        Isoelectric Point: 8.4848

>NTDB_id=76292 M2Z51_RS01210 WP_001247903.1 263276..265480(+) (priA) [Vibrio cholerae strain V130003]
MRPTIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALTGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=76292 M2Z51_RS01210 WP_001247903.1 263276..265480(+) (priA) [Vibrio cholerae strain V130003]
ATGCGACCCACCATTGCCCGAGTTTGCCTGCCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTACAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAACAAGGTTTCGGCTGTTTTTTATTAGAAGGGGTAACGGGCTCAGGTAAAACAGAGGTGTATCTTAACCTCATCACC
CCTGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACTCCACAAACCATCAATCGTTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTGATGCACTCGGCGCTCAACGACACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTATAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTCGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCACAGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGCCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTCACTTTAGTCGCGCTGTTGGATGTT
GATGGTTCACTGTATAGCAGCGATTTTCGCGCTTCTGAGCGCTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGTGAAGTGGTGTTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGCAAGCTGGCACAACTCCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAGCTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.708

100

0.44

Multiple sequence alignment