Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   OU809_RS13295 Genome accession   NZ_CP113516
Coordinates   2675569..2676603 (-) Length   344 a.a.
NCBI ID   WP_039072847.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain 285-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2670569..2681603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU809_RS13245 (OU809_13245) tasA 2670639..2671433 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  OU809_RS13250 (OU809_13250) sipW 2671506..2672090 (-) 585 WP_020452168.1 signal peptidase I SipW -
  OU809_RS13255 (OU809_13255) tapA 2672087..2672815 (-) 729 WP_268050583.1 amyloid fiber anchoring/assembly protein TapA -
  OU809_RS13260 (OU809_13260) - 2673093..2673413 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  OU809_RS13265 (OU809_13265) - 2673443..2673625 (-) 183 WP_020452171.1 YqzE family protein -
  OU809_RS13270 (OU809_13270) comGG 2673714..2674079 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  OU809_RS13275 (OU809_13275) comGF 2674091..2674579 (-) 489 WP_224067282.1 competence type IV pilus minor pilin ComGF -
  OU809_RS13280 (OU809_13280) comGE 2674488..2674835 (-) 348 WP_023855191.1 competence type IV pilus minor pilin ComGE -
  OU809_RS13285 (OU809_13285) comGD 2674819..2675256 (-) 438 WP_039072845.1 competence type IV pilus minor pilin ComGD -
  OU809_RS13290 (OU809_13290) comGC 2675262..2675555 (-) 294 WP_039072846.1 competence type IV pilus major pilin ComGC Machinery gene
  OU809_RS13295 (OU809_13295) comGB 2675569..2676603 (-) 1035 WP_039072847.1 competence type IV pilus assembly protein ComGB Machinery gene
  OU809_RS13300 (OU809_13300) comGA 2676593..2677657 (-) 1065 WP_039072848.1 competence type IV pilus ATPase ComGA Machinery gene
  OU809_RS13305 (OU809_13305) - 2677815..2678654 (-) 840 WP_039072849.1 STAS domain-containing protein -
  OU809_RS13315 (OU809_13315) - 2678896..2679273 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  OU809_RS13320 (OU809_13320) - 2679484..2679729 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  OU809_RS13325 (OU809_13325) - 2679765..2680403 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  OU809_RS13330 (OU809_13330) - 2680559..2680732 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  OU809_RS13335 (OU809_13335) - 2680793..2681107 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39818.13 Da        Isoelectric Point: 9.7341

>NTDB_id=762611 OU809_RS13295 WP_039072847.1 2675569..2676603(-) (comGB) [Bacillus paralicheniformis strain 285-3]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LTFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=762611 OU809_RS13295 WP_039072847.1 2675569..2676603(-) (comGB) [Bacillus paralicheniformis strain 285-3]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTAACATTTCATAAAAATGAAGCGAAGCGCCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538